Evaluation of the binding affinity of E3 ubiquitin ligase ligands by cellular target engagement and in-cell ELISA assay

The discovery of potent cell-permeable E3 ubiquitin ligase ligands can significantly facilitate the development of proteolysis targeting chimeras (PROTACs). Here, we present a protocol to determine the binding affinity of ligands toward CRBN E3 ubiquitin ligase, using a cellular target engagement me...

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Detalles Bibliográficos
Autores principales: Yang, Ka, Zhou, Yaxian, Roberts, Brett L., Nie, Xueqing, Tang, Weiping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829257/
https://www.ncbi.nlm.nih.gov/pubmed/33532735
http://dx.doi.org/10.1016/j.xpro.2020.100288

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829257/
https://www.ncbi.nlm.nih.gov/pubmed/33532735
http://dx.doi.org/10.1016/j.xpro.2020.100288

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