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Isolation of spermatogenic cells from the cynomolgus macaque testis with flow cytometry

Here, we describe a detailed protocol for the isolation of purified populations of viable spermatogenic cells derived from the non-human primate model organism Macaca fascicularis (cynomolgus). Using fluorescence-activated cell sorting (FACS), we describe methods to isolate spermatogonia and primary...

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Detalles Bibliográficos
Autores principales: Lau, Xianzhong, Yong, Pearly Jean Ai, Lou, Charles Kang Liang, Munusamy, Prabhakaran, Sangrithi, Mahesh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829259/
https://www.ncbi.nlm.nih.gov/pubmed/33532739
http://dx.doi.org/10.1016/j.xpro.2021.100294