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Isolation of spermatogenic cells from the cynomolgus macaque testis with flow cytometry
Here, we describe a detailed protocol for the isolation of purified populations of viable spermatogenic cells derived from the non-human primate model organism Macaca fascicularis (cynomolgus). Using fluorescence-activated cell sorting (FACS), we describe methods to isolate spermatogonia and primary...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7829259/ https://www.ncbi.nlm.nih.gov/pubmed/33532739 http://dx.doi.org/10.1016/j.xpro.2021.100294 |