Cargando…

Multiple-Monitor HPLC Assays for Rapid Process Development, In-Process Monitoring, and Validation of AAV Production and Purification

HPLC is established as a fast convenient analytical technology for characterizing the content of empty and full capsids in purified samples containing adeno-associated virus (AAV). UV-based monitoring unfortunately over-estimates the proportion of full capsids and offers little value for characteriz...

Descripción completa

Detalles Bibliográficos
Autores principales:	Gagnon, Pete, Goricar, Blaz, Mencin, Nina, Zvanut, Timotej, Peljhan, Sebastijan, Leskovec, Maja, Strancar, Ales
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7830902/ https://www.ncbi.nlm.nih.gov/pubmed/33477351 http://dx.doi.org/10.3390/pharmaceutics13010113

Ejemplares similares

High Recovery Chromatographic Purification of mRNA at Room Temperature and Neutral pH
por: Miklavčič, Rok, et al.
Publicado: (2023)

TPP Combined with DGUC as an Economic and Universal Process for Large-Scale Purification of AAV Vectors
por: Yu, Zhe, et al.
Publicado: (2019)

Purification and monitoring
por: Campanelli, M, et al.
Publicado: (2019)

HPLC Analysis and Purification of Peptides
por: Mant, Colin T., et al.
Publicado: (2007)

Immunological Monitoring to Rationally Guide AAV Gene Therapy
por: Britten, Cedrik Michael, et al.
Publicado: (2013)

Automated HPLC monitoring of broth components on bioreactors
por: Favre, Eric, et al.
Publicado: (1989)

Concentration, purification and quantification of milk protein residues following cleaning processes using a combination of SPE and RP-HPLC
por: Kürzl, Christian, et al.
Publicado: (2022)

Genetic Variability of Antioxidative Mechanisms and Cardiotoxicity after Adjuvant Radiotherapy in HER2-Positive Breast Cancer Patients
por: Marinko, Tanja, et al.
Publicado: (2020)

Quality monitoring of Shenmai injection by HPLC pharmacodynamic fingerprinting
por: Yang, Geng, et al.
Publicado: (2023)

Monitoring and Occurrence of Heavy PAHs in Pomace Oil Supply Chain Using a Double-Step Solid-Phase Purification and HPLC-FLD Determination
por: Barp, Laura, et al.
Publicado: (2022)

Universal Method for the Purification of Recombinant AAV Vectors of Differing Serotypes
por: Nass, Shelley A., et al.
Publicado: (2017)

HPLC purification technique: synthesis of unsymmetrical thiobarbituric acids
por: Deotale, Vinod D., et al.
Publicado: (2019)

Total chemical synthesis of proteins without HPLC purification
por: Loibl, S. F., et al.
Publicado: (2016)

AAV process intensification by perfusion bioreaction and integrated clarification
por: Mendes, João P., et al.
Publicado: (2022)

Process Monitoring Evaluation and Implementation for the Wood Abrasive Machining Process
por: Saloni, Daniel E., et al.
Publicado: (2010)

Multivariate Statistical Process Control: Process Monitoring Methods and Applications
por: Ge, Zhiqiang, et al.
Publicado: (2013)

Co-Extraction and Co-Purification Coupled with HPLC-DAD for Simultaneous Detection of Acrylamide and 5-hydroxymethyl-2-furfural in Thermally Processed Foods
por: Shi, Jiaqi, et al.
Publicado: (2019)

In-Line Monitoring of Downstream Purification Processes for VSV Based SARS-CoV-2 Vaccine Using a Novel Technique
por: Makovitzki, Arik, et al.
Publicado: (2021)

Advanced monitoring with complex stream processing
por: MAGNONI, Luca
Publicado: (2015)

Signal Processing for Beam Position Monitors
por: Vismara, Giuseppe
Publicado: (2000)

Prescriptive process monitoring: Quo vadis?
por: Kubrak, Kateryna, et al.
Publicado: (2022)

Development of HPLC methods for the purification and analysis of plasma membrane glycoproteins.
por: Renauer, D, et al.
Publicado: (1990)

Membranes for Gas Separation and Purification Processes
por: Chuah, Chong Yang
Publicado: (2022)

Bi-annual progress reports and meetings on purification and monitoring
por: Manenti, L.
Publicado: (2018)

Monitoring of Processing Conditions of an Ultrasonic Vibration-Assisted Ball-Burnishing Process
por: Estevez-Urra, Aida, et al.
Publicado: (2020)

Monitoring by HPLC of Chamomile Flavonoids Exposed to Rat Liver Microsomal Metabolism
por: Petroianu, Georg, et al.
Publicado: (2009)

Web service for the monitoring of dataset processing in CMS
por: Moreau, Tanguy
Publicado: (2015)

Signal processing for the CLIC beam position monitor
por: Sladen, Jonathan P H
Publicado: (1990)

Engineering Process Monitoring for Control Room Operation
por: Bätz, M
Publicado: (2001)

Lateralization of spatial information processing in response monitoring
por: Stock, Ann-Kathrin, et al.
Publicado: (2014)

Pulse Ultrasonic Cure Monitoring of the Pultrusion Process
por: Scholle, Patrick, et al.
Publicado: (2018)

On-Line Monitoring of Blind Fastener Installation Process
por: Camacho, Javier, et al.
Publicado: (2019)

Business Process Monitoring on Blockchains: Potentials and Challenges
por: Di Ciccio, Claudio, et al.
Publicado: (2020)

Ultrasound Sensors for Process Monitoring in Injection Moulding
por: Kariminejad, Mandana, et al.
Publicado: (2021)

Biological Signal Processing and Analysis for Healthcare Monitoring
por: Wu, Yunfeng, et al.
Publicado: (2022)

Monitoring Osseointegration Process Using Vibration Analysis
por: Lu, Shouxun, et al.
Publicado: (2022)

Editorial: Advances in nutrition, food processing and monitoring
por: Zaukuu, John-Lewis Zinia, et al.
Publicado: (2023)

Highly Efficient Ultracentrifugation-free Chromatographic Purification of Recombinant AAV Serotype 9
por: Tomono, Taro, et al.
Publicado: (2018)

Characterization of AAV-Specific Affinity Ligands: Consequences for Vector Purification and Development Strategies
por: Mietzsch, Mario, et al.
Publicado: (2020)

High-efficiency purification of divergent AAV serotypes using AAVX affinity chromatography
por: Florea, Michael, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_6dv7u7ovp1mcl3u5qc4270it87