Synergistic optimisation of expression, folding, and secretion improves E. coli AppA phytase production in Pichia pastoris

BACKGROUND: Pichia pastoris (Komagataella phaffii) is an important platform for heterologous protein production due to its growth to high cell density and outstanding secretory capabilities. Recent developments in synthetic biology have extended the toolbox for genetic engineering of P. pastoris to...

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Detalles Bibliográficos
Autores principales: Navone, Laura, Vogl, Thomas, Luangthongkam, Pawarisa, Blinco, Jo-Anne, Luna-Flores, Carlos, Chen, Xiaojing, von Hellens, Juhani, Speight, Robert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7836175/
https://www.ncbi.nlm.nih.gov/pubmed/33494776
http://dx.doi.org/10.1186/s12934-020-01499-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7836175/
https://www.ncbi.nlm.nih.gov/pubmed/33494776
http://dx.doi.org/10.1186/s12934-020-01499-7

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