Cancer-Related Mutations Are Not Enriched in Naive Human Pluripotent Stem Cells

Previous analysis of RNA sequencing (RNA-seq) data from human naive pluripotent stem cells reported multiple point “mutations” in cancer-related genes and implicated selective culture conditions. We observed, however, that those mutations were only present in co-cultures with mouse feeder cells. Ins...

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Detalles Bibliográficos
Autores principales: Stirparo, Giuliano Giuseppe, Smith, Austin, Guo, Ge
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2021
Materias:
Matters Arising
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837212/
https://www.ncbi.nlm.nih.gov/pubmed/33321074
http://dx.doi.org/10.1016/j.stem.2020.11.014
Descripción
Sumario:Previous analysis of RNA sequencing (RNA-seq) data from human naive pluripotent stem cells reported multiple point “mutations” in cancer-related genes and implicated selective culture conditions. We observed, however, that those mutations were only present in co-cultures with mouse feeder cells. Inspection of reads containing the polymorphisms revealed complete identity to the mouse reference genome. After we filtered reads to remove sequences of mouse origin, the actual incidence of oncogenic polymorphisms arising in naive pluripotent stem cells is close to zero.

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