Cancer-Related Mutations Are Not Enriched in Naive Human Pluripotent Stem Cells

Previous analysis of RNA sequencing (RNA-seq) data from human naive pluripotent stem cells reported multiple point “mutations” in cancer-related genes and implicated selective culture conditions. We observed, however, that those mutations were only present in co-cultures with mouse feeder cells. Ins...

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Detalles Bibliográficos
Autores principales: Stirparo, Giuliano Giuseppe, Smith, Austin, Guo, Ge
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2021
Materias:
Matters Arising
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837212/
https://www.ncbi.nlm.nih.gov/pubmed/33321074
http://dx.doi.org/10.1016/j.stem.2020.11.014
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author Stirparo, Giuliano Giuseppe
Smith, Austin
Guo, Ge
author_facet Stirparo, Giuliano Giuseppe
Smith, Austin
Guo, Ge
author_sort Stirparo, Giuliano Giuseppe
collection PubMed
description Previous analysis of RNA sequencing (RNA-seq) data from human naive pluripotent stem cells reported multiple point “mutations” in cancer-related genes and implicated selective culture conditions. We observed, however, that those mutations were only present in co-cultures with mouse feeder cells. Inspection of reads containing the polymorphisms revealed complete identity to the mouse reference genome. After we filtered reads to remove sequences of mouse origin, the actual incidence of oncogenic polymorphisms arising in naive pluripotent stem cells is close to zero.
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spelling pubmed-78372122021-02-01 Cancer-Related Mutations Are Not Enriched in Naive Human Pluripotent Stem Cells Stirparo, Giuliano Giuseppe Smith, Austin Guo, Ge Cell Stem Cell Matters Arising Previous analysis of RNA sequencing (RNA-seq) data from human naive pluripotent stem cells reported multiple point “mutations” in cancer-related genes and implicated selective culture conditions. We observed, however, that those mutations were only present in co-cultures with mouse feeder cells. Inspection of reads containing the polymorphisms revealed complete identity to the mouse reference genome. After we filtered reads to remove sequences of mouse origin, the actual incidence of oncogenic polymorphisms arising in naive pluripotent stem cells is close to zero. Cell Press 2021-01-07 /pmc/articles/PMC7837212/ /pubmed/33321074 http://dx.doi.org/10.1016/j.stem.2020.11.014 Text en © 2020 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Matters Arising
Stirparo, Giuliano Giuseppe
Smith, Austin
Guo, Ge
Cancer-Related Mutations Are Not Enriched in Naive Human Pluripotent Stem Cells
title Cancer-Related Mutations Are Not Enriched in Naive Human Pluripotent Stem Cells
title_full Cancer-Related Mutations Are Not Enriched in Naive Human Pluripotent Stem Cells
title_fullStr Cancer-Related Mutations Are Not Enriched in Naive Human Pluripotent Stem Cells
title_full_unstemmed Cancer-Related Mutations Are Not Enriched in Naive Human Pluripotent Stem Cells
title_short Cancer-Related Mutations Are Not Enriched in Naive Human Pluripotent Stem Cells
title_sort cancer-related mutations are not enriched in naive human pluripotent stem cells
topic Matters Arising
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7837212/
https://www.ncbi.nlm.nih.gov/pubmed/33321074
http://dx.doi.org/10.1016/j.stem.2020.11.014
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