Cargando…

miR-183 Modulates Cell Apoptosis and Proliferation in Tongue Squamous Cell Carcinoma SCC25 Cell Line

This study was designed to investigate the role of miR-183 in modulating cell growth and apoptosis of tongue squamous cell carcinoma SCC25 cell line. Human squamous epithelial cell and squamous cell carcinoma cell line SCC25 was used, and miR-183 was inhibited. Cell growth, colony formation, and apo...

Descripción completa

Detalles Bibliográficos
Autores principales: Yan, Dayong, Cai, Xiaoqing, Feng, Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cognizant Communication Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7838737/
https://www.ncbi.nlm.nih.gov/pubmed/28281960
http://dx.doi.org/10.3727/096504016X14685034103239
_version_ 1783643249372561408
author Yan, Dayong
Cai, Xiaoqing
Feng, Yu
author_facet Yan, Dayong
Cai, Xiaoqing
Feng, Yu
author_sort Yan, Dayong
collection PubMed
description This study was designed to investigate the role of miR-183 in modulating cell growth and apoptosis of tongue squamous cell carcinoma SCC25 cell line. Human squamous epithelial cell and squamous cell carcinoma cell line SCC25 was used, and miR-183 was inhibited. Cell growth, colony formation, and apoptotic rate, as well as the expression of caspase 3 and BCL-xL, were detected. Results showed that miR-183 was significantly overexpressed in the SCC25 cell line when compared with normal control. The miR-183 inhibitor reduced cell growth and colony formation, while the apoptosis percentage was significantly increased. The expression of activated caspase 3 and BCL-xL was obviously up- and downregulated in siRNA-transfected cells, respectively. In conclusion, miR-183 contributed to cell growth and proliferation, and suppressed cell apoptosis in SCC25 cells. Therefore, miR-183 might serve as a therapeutic target in tongue squamous cell carcinoma (TSCC).
format Online
Article
Text
id pubmed-7838737
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Cognizant Communication Corporation
record_format MEDLINE/PubMed
spelling pubmed-78387372021-02-16 miR-183 Modulates Cell Apoptosis and Proliferation in Tongue Squamous Cell Carcinoma SCC25 Cell Line Yan, Dayong Cai, Xiaoqing Feng, Yu Oncol Res Article This study was designed to investigate the role of miR-183 in modulating cell growth and apoptosis of tongue squamous cell carcinoma SCC25 cell line. Human squamous epithelial cell and squamous cell carcinoma cell line SCC25 was used, and miR-183 was inhibited. Cell growth, colony formation, and apoptotic rate, as well as the expression of caspase 3 and BCL-xL, were detected. Results showed that miR-183 was significantly overexpressed in the SCC25 cell line when compared with normal control. The miR-183 inhibitor reduced cell growth and colony formation, while the apoptosis percentage was significantly increased. The expression of activated caspase 3 and BCL-xL was obviously up- and downregulated in siRNA-transfected cells, respectively. In conclusion, miR-183 contributed to cell growth and proliferation, and suppressed cell apoptosis in SCC25 cells. Therefore, miR-183 might serve as a therapeutic target in tongue squamous cell carcinoma (TSCC). Cognizant Communication Corporation 2016-10-27 /pmc/articles/PMC7838737/ /pubmed/28281960 http://dx.doi.org/10.3727/096504016X14685034103239 Text en Copyright © 2016 Cognizant, LLC. http://creativecommons.org/licenses/by-nc-nd/4.0/ This article is licensed under a Creative Commons Attribution-NonCommercial NoDerivatives 4.0 International License.
spellingShingle Article
Yan, Dayong
Cai, Xiaoqing
Feng, Yu
miR-183 Modulates Cell Apoptosis and Proliferation in Tongue Squamous Cell Carcinoma SCC25 Cell Line
title miR-183 Modulates Cell Apoptosis and Proliferation in Tongue Squamous Cell Carcinoma SCC25 Cell Line
title_full miR-183 Modulates Cell Apoptosis and Proliferation in Tongue Squamous Cell Carcinoma SCC25 Cell Line
title_fullStr miR-183 Modulates Cell Apoptosis and Proliferation in Tongue Squamous Cell Carcinoma SCC25 Cell Line
title_full_unstemmed miR-183 Modulates Cell Apoptosis and Proliferation in Tongue Squamous Cell Carcinoma SCC25 Cell Line
title_short miR-183 Modulates Cell Apoptosis and Proliferation in Tongue Squamous Cell Carcinoma SCC25 Cell Line
title_sort mir-183 modulates cell apoptosis and proliferation in tongue squamous cell carcinoma scc25 cell line
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7838737/
https://www.ncbi.nlm.nih.gov/pubmed/28281960
http://dx.doi.org/10.3727/096504016X14685034103239
work_keys_str_mv AT yandayong mir183modulatescellapoptosisandproliferationintonguesquamouscellcarcinomascc25cellline
AT caixiaoqing mir183modulatescellapoptosisandproliferationintonguesquamouscellcarcinomascc25cellline
AT fengyu mir183modulatescellapoptosisandproliferationintonguesquamouscellcarcinomascc25cellline