SARS-CoV-2 3a expression, purification, and reconstitution into lipid nanodiscs

The SARS-CoV-2 3a protein is a putative ion channel implicated in virus life cycle and pathogenesis. We recently expressed, purified, and reconstituted 3a into lipid nanodiscs to solve its structure by cryo-EM to 2.1 Å resolution. In this chapter, we describe methods we developed in order to facilit...

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Detalles Bibliográficos
Autores principales: Kern, David M., Brohawn, Stephen G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7839409/
https://www.ncbi.nlm.nih.gov/pubmed/34099172
http://dx.doi.org/10.1016/bs.mie.2020.12.020

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7839409/
https://www.ncbi.nlm.nih.gov/pubmed/34099172
http://dx.doi.org/10.1016/bs.mie.2020.12.020

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