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SARS-CoV-2 3a expression, purification, and reconstitution into lipid nanodiscs
The SARS-CoV-2 3a protein is a putative ion channel implicated in virus life cycle and pathogenesis. We recently expressed, purified, and reconstituted 3a into lipid nanodiscs to solve its structure by cryo-EM to 2.1 Å resolution. In this chapter, we describe methods we developed in order to facilit...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Elsevier Inc.
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7839409/ https://www.ncbi.nlm.nih.gov/pubmed/34099172 http://dx.doi.org/10.1016/bs.mie.2020.12.020 |