Engineering Regioselectivity of a P450 Monooxygenase Enables the Synthesis of Ursodeoxycholic Acid via 7β‐Hydroxylation of Lithocholic Acid

We engineered the cytochrome P450 monooxygenase CYP107D1 (OleP) from Streptomyces antibioticus for the stereo‐ and regioselective 7β‐hydroxylation of lithocholic acid (LCA) to yield ursodeoxycholic acid (UDCA). OleP was previously shown to hydroxylate testosterone at the 7β‐position but LCA is exclu...

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Detalles Bibliográficos
Autores principales: Grobe, Sascha, Badenhorst, Christoffel P. S., Bayer, Thomas, Hamnevik, Emil, Wu, Shuke, Grathwol, Christoph W., Link, Andreas, Koban, Sven, Brundiek, Henrike, Großjohann, Beatrice, Bornscheuer, Uwe T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7839452/
https://www.ncbi.nlm.nih.gov/pubmed/33085147
http://dx.doi.org/10.1002/anie.202012675

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7839452/
https://www.ncbi.nlm.nih.gov/pubmed/33085147
http://dx.doi.org/10.1002/anie.202012675

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