A Wnt Pathway Activator Induces Apoptosis and Cell Death in Mouse Monocytic Leukemia Cells

A Wnt agonist, 2-amino-4-[3,4-(methylenedioxy)benzylamino]-6-(3-methoxyphenyl) pyrimidine, is a cell-permeable pyrimidine compound that has been shown to mimic the effect of Wnt. In this study, leukemic mouse cell lines, RAW 264.7 and J774.1, were incubated with the Wnt agonist. The Wnt agonist show...

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Autores principales: Kato, Yoshiro, Naiki, Yoshikazu, Komatsu, Takayuki, Takahashi, Kazuko, Nakamura, Jiro, Koide, Naoki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cognizant Communication Corporation 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7841171/
https://www.ncbi.nlm.nih.gov/pubmed/27623760
http://dx.doi.org/10.3727/096504016X14721731148893
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author Kato, Yoshiro
Naiki, Yoshikazu
Komatsu, Takayuki
Takahashi, Kazuko
Nakamura, Jiro
Koide, Naoki
author_facet Kato, Yoshiro
Naiki, Yoshikazu
Komatsu, Takayuki
Takahashi, Kazuko
Nakamura, Jiro
Koide, Naoki
author_sort Kato, Yoshiro
collection PubMed
description A Wnt agonist, 2-amino-4-[3,4-(methylenedioxy)benzylamino]-6-(3-methoxyphenyl) pyrimidine, is a cell-permeable pyrimidine compound that has been shown to mimic the effect of Wnt. In this study, leukemic mouse cell lines, RAW 264.7 and J774.1, were incubated with the Wnt agonist. The Wnt agonist showed cell death in the concentration of 1–10 μM. The Wnt agonist did not show inhibition of GSK-3β activity but induced β-catenin accumulation in the nucleus. The Wnt agonist showed caspase-independent cell death, but no further involvement in cell death ER stress signaling. Here we discuss the possible mechanism of Wnt agonist-induced apoptotic cell death in RAW 264.7 cells.
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spelling pubmed-78411712021-02-16 A Wnt Pathway Activator Induces Apoptosis and Cell Death in Mouse Monocytic Leukemia Cells Kato, Yoshiro Naiki, Yoshikazu Komatsu, Takayuki Takahashi, Kazuko Nakamura, Jiro Koide, Naoki Oncol Res Article A Wnt agonist, 2-amino-4-[3,4-(methylenedioxy)benzylamino]-6-(3-methoxyphenyl) pyrimidine, is a cell-permeable pyrimidine compound that has been shown to mimic the effect of Wnt. In this study, leukemic mouse cell lines, RAW 264.7 and J774.1, were incubated with the Wnt agonist. The Wnt agonist showed cell death in the concentration of 1–10 μM. The Wnt agonist did not show inhibition of GSK-3β activity but induced β-catenin accumulation in the nucleus. The Wnt agonist showed caspase-independent cell death, but no further involvement in cell death ER stress signaling. Here we discuss the possible mechanism of Wnt agonist-induced apoptotic cell death in RAW 264.7 cells. Cognizant Communication Corporation 2017-04-14 /pmc/articles/PMC7841171/ /pubmed/27623760 http://dx.doi.org/10.3727/096504016X14721731148893 Text en Copyright © 2017 Cognizant, LLC. http://creativecommons.org/licenses/by-nc-nd/4.0/ This article is licensed under a Creative Commons Attribution-NonCommercial NoDerivatives 4.0 International License.
spellingShingle Article
Kato, Yoshiro
Naiki, Yoshikazu
Komatsu, Takayuki
Takahashi, Kazuko
Nakamura, Jiro
Koide, Naoki
A Wnt Pathway Activator Induces Apoptosis and Cell Death in Mouse Monocytic Leukemia Cells
title A Wnt Pathway Activator Induces Apoptosis and Cell Death in Mouse Monocytic Leukemia Cells
title_full A Wnt Pathway Activator Induces Apoptosis and Cell Death in Mouse Monocytic Leukemia Cells
title_fullStr A Wnt Pathway Activator Induces Apoptosis and Cell Death in Mouse Monocytic Leukemia Cells
title_full_unstemmed A Wnt Pathway Activator Induces Apoptosis and Cell Death in Mouse Monocytic Leukemia Cells
title_short A Wnt Pathway Activator Induces Apoptosis and Cell Death in Mouse Monocytic Leukemia Cells
title_sort wnt pathway activator induces apoptosis and cell death in mouse monocytic leukemia cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7841171/
https://www.ncbi.nlm.nih.gov/pubmed/27623760
http://dx.doi.org/10.3727/096504016X14721731148893
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