Epigenetic silencing directs expression heterogeneity of stably integrated multi-transcript unit genetic circuits

We report that epigenetic silencing causes the loss of function of multi-transcript unit constructs that are integrated using CRISPR-Cas9. Using a modular two color reporter system flanked by selection markers, we demonstrate that expression heterogeneity does not correlate with sequence alteration...

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Detalles Bibliográficos
Autores principales: Zimak, Jan, Wagoner, Zachary W., Nelson, Nellie, Waechtler, Brooke, Schlosser, Hana, Kopecky, Morgan, Wu, Jie, Zhao, Weian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844226/
https://www.ncbi.nlm.nih.gov/pubmed/33510302
http://dx.doi.org/10.1038/s41598-021-81975-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844226/
https://www.ncbi.nlm.nih.gov/pubmed/33510302
http://dx.doi.org/10.1038/s41598-021-81975-1

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