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Epigenetic silencing directs expression heterogeneity of stably integrated multi-transcript unit genetic circuits
We report that epigenetic silencing causes the loss of function of multi-transcript unit constructs that are integrated using CRISPR-Cas9. Using a modular two color reporter system flanked by selection markers, we demonstrate that expression heterogeneity does not correlate with sequence alteration...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844226/ https://www.ncbi.nlm.nih.gov/pubmed/33510302 http://dx.doi.org/10.1038/s41598-021-81975-1 |