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Multi-locus sequence typing and virulence profile in Bacillus cereus sensu lato strains isolated from dairy products

Members of Bacillus cereus group are important food contaminants and they are of relevant interest in food safety and public heath due to their ability to cause two distinct forms of food poisoning, emetic and diarrhoeal syndrome. In the present study, 90 strains of B. cereus isolated from dairy pro...

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Autores principales: Bianco, Angelica, Capozzi, Loredana, Miccolupo, Angela, Iannetti, Simona, Danzetta, Maria Luisa, Del Sambro, Laura, Caruso, Marta, Santagada, Gianfranco, Parisi, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PAGEPress Publications, Pavia, Italy 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844584/
https://www.ncbi.nlm.nih.gov/pubmed/33532366
http://dx.doi.org/10.4081/ijfs.2020.8401
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author Bianco, Angelica
Capozzi, Loredana
Miccolupo, Angela
Iannetti, Simona
Danzetta, Maria Luisa
Del Sambro, Laura
Caruso, Marta
Santagada, Gianfranco
Parisi, Antonio
author_facet Bianco, Angelica
Capozzi, Loredana
Miccolupo, Angela
Iannetti, Simona
Danzetta, Maria Luisa
Del Sambro, Laura
Caruso, Marta
Santagada, Gianfranco
Parisi, Antonio
author_sort Bianco, Angelica
collection PubMed
description Members of Bacillus cereus group are important food contaminants and they are of relevant interest in food safety and public heath due to their ability to cause two distinct forms of food poisoning, emetic and diarrhoeal syndrome. In the present study, 90 strains of B. cereus isolated from dairy products, have been typed using Multilocus Sequence Typing (MLST) analysis and investigated for the occurrence of 10 enterotoxigenic genes (hblA, hblC, hblD, nheA, nheB, nheC, cytK, entFM, entS and bceT) and one emetogenic gene (ces), to determine their genetic diversity. A total of 58 sequence types were identified and among these 17 were signalled as new profiles. Among the virulence genes, the majority of our strains carried the entS (92%), entFM (86%), nhe (82%) and cytK (72%) genes. All remaining genes were identified in at least one strain with different prevalence, stressing the genetic diversity, how even the different grade of pathogenicity of B. cereus isolated from dairy products.
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spelling pubmed-78445842021-02-01 Multi-locus sequence typing and virulence profile in Bacillus cereus sensu lato strains isolated from dairy products Bianco, Angelica Capozzi, Loredana Miccolupo, Angela Iannetti, Simona Danzetta, Maria Luisa Del Sambro, Laura Caruso, Marta Santagada, Gianfranco Parisi, Antonio Ital J Food Saf Article Members of Bacillus cereus group are important food contaminants and they are of relevant interest in food safety and public heath due to their ability to cause two distinct forms of food poisoning, emetic and diarrhoeal syndrome. In the present study, 90 strains of B. cereus isolated from dairy products, have been typed using Multilocus Sequence Typing (MLST) analysis and investigated for the occurrence of 10 enterotoxigenic genes (hblA, hblC, hblD, nheA, nheB, nheC, cytK, entFM, entS and bceT) and one emetogenic gene (ces), to determine their genetic diversity. A total of 58 sequence types were identified and among these 17 were signalled as new profiles. Among the virulence genes, the majority of our strains carried the entS (92%), entFM (86%), nhe (82%) and cytK (72%) genes. All remaining genes were identified in at least one strain with different prevalence, stressing the genetic diversity, how even the different grade of pathogenicity of B. cereus isolated from dairy products. PAGEPress Publications, Pavia, Italy 2021-01-18 /pmc/articles/PMC7844584/ /pubmed/33532366 http://dx.doi.org/10.4081/ijfs.2020.8401 Text en ©Copyright: the Author(s) http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Bianco, Angelica
Capozzi, Loredana
Miccolupo, Angela
Iannetti, Simona
Danzetta, Maria Luisa
Del Sambro, Laura
Caruso, Marta
Santagada, Gianfranco
Parisi, Antonio
Multi-locus sequence typing and virulence profile in Bacillus cereus sensu lato strains isolated from dairy products
title Multi-locus sequence typing and virulence profile in Bacillus cereus sensu lato strains isolated from dairy products
title_full Multi-locus sequence typing and virulence profile in Bacillus cereus sensu lato strains isolated from dairy products
title_fullStr Multi-locus sequence typing and virulence profile in Bacillus cereus sensu lato strains isolated from dairy products
title_full_unstemmed Multi-locus sequence typing and virulence profile in Bacillus cereus sensu lato strains isolated from dairy products
title_short Multi-locus sequence typing and virulence profile in Bacillus cereus sensu lato strains isolated from dairy products
title_sort multi-locus sequence typing and virulence profile in bacillus cereus sensu lato strains isolated from dairy products
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7844584/
https://www.ncbi.nlm.nih.gov/pubmed/33532366
http://dx.doi.org/10.4081/ijfs.2020.8401
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