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Genetic characterization of an almond germplasm collection and volatilome profiling of raw and roasted kernels

Almond is appreciated for its nutraceutical value and for the aromatic profile of the kernels. In this work, an almond collection composed of 96 Sicilian accessions complemented with 10 widely cultivated cultivars was phenotyped for the production of volatile organic compounds using a proton-transfe...

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Autores principales: Di Guardo, M., Farneti, B., Khomenko, I., Modica, G., Mosca, A., Distefano, G., Bianco, L., Troggio, M., Sottile, F., La Malfa, S., Biasioli, F., Gentile, A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7848010/
https://www.ncbi.nlm.nih.gov/pubmed/33518710
http://dx.doi.org/10.1038/s41438-021-00465-7
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author Di Guardo, M.
Farneti, B.
Khomenko, I.
Modica, G.
Mosca, A.
Distefano, G.
Bianco, L.
Troggio, M.
Sottile, F.
La Malfa, S.
Biasioli, F.
Gentile, A.
author_facet Di Guardo, M.
Farneti, B.
Khomenko, I.
Modica, G.
Mosca, A.
Distefano, G.
Bianco, L.
Troggio, M.
Sottile, F.
La Malfa, S.
Biasioli, F.
Gentile, A.
author_sort Di Guardo, M.
collection PubMed
description Almond is appreciated for its nutraceutical value and for the aromatic profile of the kernels. In this work, an almond collection composed of 96 Sicilian accessions complemented with 10 widely cultivated cultivars was phenotyped for the production of volatile organic compounds using a proton-transfer time-of-flight mass spectrometer and genotyped using the Illumina Infinium(®)18 K Peach SNP array. The profiling of the aroma was carried out on fresh and roasted kernels enabling the detection of 150 mass peaks. Sixty eight, for the most related with sulfur compounds, furan containing compounds, and aldehydes formed by Strecker degradation, significantly increased during roasting, while the concentration of fifty-four mass peaks, for the most belonging to alcohols and terpenes, significantly decreased. Four hundred and seventy-one robust SNPs were selected and employed for population genetic studies. Structure analysis detected three subpopulations with the Sicilian accessions characterized by a different genetic stratification compared to those collected in Apulia (South Italy) and the International cultivars. The linkage-disequilibrium (LD) decay across the genome was equal to r(2) = 0.083. Furthermore, a high level of collinearity (r(2) = 0.96) between almond and peach was registered confirming the high synteny between the two genomes. A preliminary application of a genome-wide association analysis allowed the detection of significant marker-trait associations for 31 fresh and 33 roasted almond mass peaks respectively. An accurate genetic and phenotypic characterization of novel germplasm can represent a valuable tool for the set-up of marker-assisted selection of novel cultivars with an enhanced aromatic profile.
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spelling pubmed-78480102021-02-08 Genetic characterization of an almond germplasm collection and volatilome profiling of raw and roasted kernels Di Guardo, M. Farneti, B. Khomenko, I. Modica, G. Mosca, A. Distefano, G. Bianco, L. Troggio, M. Sottile, F. La Malfa, S. Biasioli, F. Gentile, A. Hortic Res Article Almond is appreciated for its nutraceutical value and for the aromatic profile of the kernels. In this work, an almond collection composed of 96 Sicilian accessions complemented with 10 widely cultivated cultivars was phenotyped for the production of volatile organic compounds using a proton-transfer time-of-flight mass spectrometer and genotyped using the Illumina Infinium(®)18 K Peach SNP array. The profiling of the aroma was carried out on fresh and roasted kernels enabling the detection of 150 mass peaks. Sixty eight, for the most related with sulfur compounds, furan containing compounds, and aldehydes formed by Strecker degradation, significantly increased during roasting, while the concentration of fifty-four mass peaks, for the most belonging to alcohols and terpenes, significantly decreased. Four hundred and seventy-one robust SNPs were selected and employed for population genetic studies. Structure analysis detected three subpopulations with the Sicilian accessions characterized by a different genetic stratification compared to those collected in Apulia (South Italy) and the International cultivars. The linkage-disequilibrium (LD) decay across the genome was equal to r(2) = 0.083. Furthermore, a high level of collinearity (r(2) = 0.96) between almond and peach was registered confirming the high synteny between the two genomes. A preliminary application of a genome-wide association analysis allowed the detection of significant marker-trait associations for 31 fresh and 33 roasted almond mass peaks respectively. An accurate genetic and phenotypic characterization of novel germplasm can represent a valuable tool for the set-up of marker-assisted selection of novel cultivars with an enhanced aromatic profile. Nature Publishing Group UK 2021-02-01 /pmc/articles/PMC7848010/ /pubmed/33518710 http://dx.doi.org/10.1038/s41438-021-00465-7 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Di Guardo, M.
Farneti, B.
Khomenko, I.
Modica, G.
Mosca, A.
Distefano, G.
Bianco, L.
Troggio, M.
Sottile, F.
La Malfa, S.
Biasioli, F.
Gentile, A.
Genetic characterization of an almond germplasm collection and volatilome profiling of raw and roasted kernels
title Genetic characterization of an almond germplasm collection and volatilome profiling of raw and roasted kernels
title_full Genetic characterization of an almond germplasm collection and volatilome profiling of raw and roasted kernels
title_fullStr Genetic characterization of an almond germplasm collection and volatilome profiling of raw and roasted kernels
title_full_unstemmed Genetic characterization of an almond germplasm collection and volatilome profiling of raw and roasted kernels
title_short Genetic characterization of an almond germplasm collection and volatilome profiling of raw and roasted kernels
title_sort genetic characterization of an almond germplasm collection and volatilome profiling of raw and roasted kernels
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7848010/
https://www.ncbi.nlm.nih.gov/pubmed/33518710
http://dx.doi.org/10.1038/s41438-021-00465-7
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