Topoisomerase I prevents transcription-replication conflicts at transcription termination sites

R-loops have both positive and negative impacts on chromosome functions. To identify toxic R-loops, we mapped RNA:DNA hybrids, markers of replication fork stalling and DNA double-strand breaks along the human genome. This analysis indicates that transient replication fork pausing occurs at the trans...

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Detalles Bibliográficos
Autores principales: Liu, Yaqun, Lin, Yea-Lih, Pasero, Philippe, Chen, Chun-Long
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2020
Materias:
Author’s Views
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7849740/
https://www.ncbi.nlm.nih.gov/pubmed/33553603
http://dx.doi.org/10.1080/23723556.2020.1843951
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author Liu, Yaqun
Lin, Yea-Lih
Pasero, Philippe
Chen, Chun-Long
author_facet Liu, Yaqun
Lin, Yea-Lih
Pasero, Philippe
Chen, Chun-Long
author_sort Liu, Yaqun
collection PubMed
description R-loops have both positive and negative impacts on chromosome functions. To identify toxic R-loops, we mapped RNA:DNA hybrids, markers of replication fork stalling and DNA double-strand breaks along the human genome. This analysis indicates that transient replication fork pausing occurs at the transcription termination sites of highly expressed genes enriched in R-loops and prevents head-on conflicts with transcription, in a topoisomerase I-dependent manner.
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spelling pubmed-78497402021-02-05 Topoisomerase I prevents transcription-replication conflicts at transcription termination sites Liu, Yaqun Lin, Yea-Lih Pasero, Philippe Chen, Chun-Long Mol Cell Oncol Author’s Views R-loops have both positive and negative impacts on chromosome functions. To identify toxic R-loops, we mapped RNA:DNA hybrids, markers of replication fork stalling and DNA double-strand breaks along the human genome. This analysis indicates that transient replication fork pausing occurs at the transcription termination sites of highly expressed genes enriched in R-loops and prevents head-on conflicts with transcription, in a topoisomerase I-dependent manner. Taylor & Francis 2020-12-07 /pmc/articles/PMC7849740/ /pubmed/33553603 http://dx.doi.org/10.1080/23723556.2020.1843951 Text en © 2020 The Author(s). Published with license by Taylor & Francis Group, LLC. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.
spellingShingle Author’s Views
Liu, Yaqun
Lin, Yea-Lih
Pasero, Philippe
Chen, Chun-Long
Topoisomerase I prevents transcription-replication conflicts at transcription termination sites
title Topoisomerase I prevents transcription-replication conflicts at transcription termination sites
title_full Topoisomerase I prevents transcription-replication conflicts at transcription termination sites
title_fullStr Topoisomerase I prevents transcription-replication conflicts at transcription termination sites
title_full_unstemmed Topoisomerase I prevents transcription-replication conflicts at transcription termination sites
title_short Topoisomerase I prevents transcription-replication conflicts at transcription termination sites
title_sort topoisomerase i prevents transcription-replication conflicts at transcription termination sites
topic Author’s Views
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7849740/
https://www.ncbi.nlm.nih.gov/pubmed/33553603
http://dx.doi.org/10.1080/23723556.2020.1843951
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AT chenchunlong topoisomeraseipreventstranscriptionreplicationconflictsattranscriptionterminationsites

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