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NuMA interaction with chromatin is vital for proper chromosome decondensation at the mitotic exit
NuMA is an abundant long coiled-coil protein that plays a prominent role in spindle organization during mitosis. In interphase, NuMA is localized to the nucleus and hypothesized to control gene expression and chromatin organization. However, because of the prominent mitotic phenotype upon NuMA loss,...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7851854/ https://www.ncbi.nlm.nih.gov/pubmed/32845810 http://dx.doi.org/10.1091/mbc.E20-06-0415 |
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author | Rajeevan, Ashwathi Keshri, Riya Kapoor, Sukriti Kotak, Sachin |
author_facet | Rajeevan, Ashwathi Keshri, Riya Kapoor, Sukriti Kotak, Sachin |
author_sort | Rajeevan, Ashwathi |
collection | PubMed |
description | NuMA is an abundant long coiled-coil protein that plays a prominent role in spindle organization during mitosis. In interphase, NuMA is localized to the nucleus and hypothesized to control gene expression and chromatin organization. However, because of the prominent mitotic phenotype upon NuMA loss, its precise function in the interphase nucleus remains elusive. Here, we report that NuMA is associated with chromatin in interphase and prophase but released upon nuclear envelope breakdown (NEBD) by the action of Cdk1. We uncover that NuMA directly interacts with DNA via evolutionarily conserved sequences in its C-terminus. Notably, the expression of the DNA-binding–deficient mutant of NuMA affects chromatin decondensation at the mitotic exit, and nuclear shape in interphase. We show that the nuclear shape defects observed upon mutant NuMA expression are due to its potential to polymerize into higher-order fibrillar structures. Overall, this work establishes the spindle-independent function of NuMA in choreographing proper chromatin decompaction and nuclear shape by directly associating with the DNA. |
format | Online Article Text |
id | pubmed-7851854 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-78518542021-02-10 NuMA interaction with chromatin is vital for proper chromosome decondensation at the mitotic exit Rajeevan, Ashwathi Keshri, Riya Kapoor, Sukriti Kotak, Sachin Mol Biol Cell Articles NuMA is an abundant long coiled-coil protein that plays a prominent role in spindle organization during mitosis. In interphase, NuMA is localized to the nucleus and hypothesized to control gene expression and chromatin organization. However, because of the prominent mitotic phenotype upon NuMA loss, its precise function in the interphase nucleus remains elusive. Here, we report that NuMA is associated with chromatin in interphase and prophase but released upon nuclear envelope breakdown (NEBD) by the action of Cdk1. We uncover that NuMA directly interacts with DNA via evolutionarily conserved sequences in its C-terminus. Notably, the expression of the DNA-binding–deficient mutant of NuMA affects chromatin decondensation at the mitotic exit, and nuclear shape in interphase. We show that the nuclear shape defects observed upon mutant NuMA expression are due to its potential to polymerize into higher-order fibrillar structures. Overall, this work establishes the spindle-independent function of NuMA in choreographing proper chromatin decompaction and nuclear shape by directly associating with the DNA. The American Society for Cell Biology 2020-10-15 /pmc/articles/PMC7851854/ /pubmed/32845810 http://dx.doi.org/10.1091/mbc.E20-06-0415 Text en © 2020 Rajeevan et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. http://creativecommons.org/licenses/by-nc-sa/3.0 This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License. |
spellingShingle | Articles Rajeevan, Ashwathi Keshri, Riya Kapoor, Sukriti Kotak, Sachin NuMA interaction with chromatin is vital for proper chromosome decondensation at the mitotic exit |
title | NuMA interaction with chromatin is vital for proper chromosome decondensation at the mitotic exit |
title_full | NuMA interaction with chromatin is vital for proper chromosome decondensation at the mitotic exit |
title_fullStr | NuMA interaction with chromatin is vital for proper chromosome decondensation at the mitotic exit |
title_full_unstemmed | NuMA interaction with chromatin is vital for proper chromosome decondensation at the mitotic exit |
title_short | NuMA interaction with chromatin is vital for proper chromosome decondensation at the mitotic exit |
title_sort | numa interaction with chromatin is vital for proper chromosome decondensation at the mitotic exit |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7851854/ https://www.ncbi.nlm.nih.gov/pubmed/32845810 http://dx.doi.org/10.1091/mbc.E20-06-0415 |
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