Strand-Specific Reverse Transcription PCR for Detection of Replicating SARS-CoV-2

We developed an assay that detects minus-strand RNA as a surrogate for actively replicating severe acute respiratory syndrome coronavirus 2. We detected minus-strand RNA in 41 persons with coronavirus disease up to 30 days after symptom onset. This assay might inform clinical decision-making about p...

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Autores principales: Hogan, Catherine A., Huang, ChunHong, Sahoo, Malaya K., Wang, Hannah, Jiang, Becky, Sibai, Mamdouh, Holubar, Marisa, Mathew, Roshni, Zehnder, James, Pinsky, Benjamin A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2021
Materias:
Dispatch
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7853532/
https://www.ncbi.nlm.nih.gov/pubmed/33496233
http://dx.doi.org/10.3201/eid2702.204168
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author Hogan, Catherine A.
Huang, ChunHong
Sahoo, Malaya K.
Wang, Hannah
Jiang, Becky
Sibai, Mamdouh
Holubar, Marisa
Mathew, Roshni
Zehnder, James
Pinsky, Benjamin A.
author_facet Hogan, Catherine A.
Huang, ChunHong
Sahoo, Malaya K.
Wang, Hannah
Jiang, Becky
Sibai, Mamdouh
Holubar, Marisa
Mathew, Roshni
Zehnder, James
Pinsky, Benjamin A.
author_sort Hogan, Catherine A.
collection PubMed
description We developed an assay that detects minus-strand RNA as a surrogate for actively replicating severe acute respiratory syndrome coronavirus 2. We detected minus-strand RNA in 41 persons with coronavirus disease up to 30 days after symptom onset. This assay might inform clinical decision-making about patient infectiousness.
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spelling pubmed-78535322021-02-09 Strand-Specific Reverse Transcription PCR for Detection of Replicating SARS-CoV-2 Hogan, Catherine A. Huang, ChunHong Sahoo, Malaya K. Wang, Hannah Jiang, Becky Sibai, Mamdouh Holubar, Marisa Mathew, Roshni Zehnder, James Pinsky, Benjamin A. Emerg Infect Dis Dispatch We developed an assay that detects minus-strand RNA as a surrogate for actively replicating severe acute respiratory syndrome coronavirus 2. We detected minus-strand RNA in 41 persons with coronavirus disease up to 30 days after symptom onset. This assay might inform clinical decision-making about patient infectiousness. Centers for Disease Control and Prevention 2021-02 /pmc/articles/PMC7853532/ /pubmed/33496233 http://dx.doi.org/10.3201/eid2702.204168 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Dispatch
Hogan, Catherine A.
Huang, ChunHong
Sahoo, Malaya K.
Wang, Hannah
Jiang, Becky
Sibai, Mamdouh
Holubar, Marisa
Mathew, Roshni
Zehnder, James
Pinsky, Benjamin A.
Strand-Specific Reverse Transcription PCR for Detection of Replicating SARS-CoV-2
title Strand-Specific Reverse Transcription PCR for Detection of Replicating SARS-CoV-2
title_full Strand-Specific Reverse Transcription PCR for Detection of Replicating SARS-CoV-2
title_fullStr Strand-Specific Reverse Transcription PCR for Detection of Replicating SARS-CoV-2
title_full_unstemmed Strand-Specific Reverse Transcription PCR for Detection of Replicating SARS-CoV-2
title_short Strand-Specific Reverse Transcription PCR for Detection of Replicating SARS-CoV-2
title_sort strand-specific reverse transcription pcr for detection of replicating sars-cov-2
topic Dispatch
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7853532/
https://www.ncbi.nlm.nih.gov/pubmed/33496233
http://dx.doi.org/10.3201/eid2702.204168
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