Optimization of AsCas12a for combinatorial genetic screens in human cells

Cas12a RNA-guided endonucleases are promising tools for multiplexed genetic perturbations because they can process multiple guide RNAs expressed as a single transcript and subsequently cleave target DNA. However, their widespread adoption has lagged behind Cas9-based strategies due to low activity a...

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Detalles Bibliográficos
Autores principales: DeWeirdt, Peter C, Sanson, Kendall R, Sangree, Annabel K, Hegde, Mudra, Hanna, Ruth E, Feeley, Marissa N, Griffith, Audrey L, Teng, Teng, Borys, Samantha M, Strand, Christine, Joung, J Keith, Kleinstiver, Benjamin P, Pan, Xuewen, Huang, Alan, Doench, John G
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7854777/
https://www.ncbi.nlm.nih.gov/pubmed/32661438
http://dx.doi.org/10.1038/s41587-020-0600-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7854777/
https://www.ncbi.nlm.nih.gov/pubmed/32661438
http://dx.doi.org/10.1038/s41587-020-0600-6

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