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Protocol for the determination of intracellular phase separation thresholds
To date, phase separation studies have largely been limited to in vitro assays using non-native conditions and aggregation-prone recombinant proteins that are often difficult to purify. This protocol describes the determination of relative protein concentration thresholds for phase separation throug...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7856470/ https://www.ncbi.nlm.nih.gov/pubmed/33554143 http://dx.doi.org/10.1016/j.xpro.2021.100308 |