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Extracting meaningful circuit-based calcium dynamics in astrocytes and neurons from adult mouse brain slices using single-photon GCaMP imaging

Confocal, multiphoton, or other advanced microscopy techniques produce high-quality datasets of calcium activity in live tissue. However, researchers without access to such expensive equipment can still produce meaningful observations from single-photon datasets. Here, we describe a protocol to extr...

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Detalles Bibliográficos
Autores principales: Asrican, Brent, Song, Juan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7856475/
https://www.ncbi.nlm.nih.gov/pubmed/33554141
http://dx.doi.org/10.1016/j.xpro.2021.100306