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Extracting meaningful circuit-based calcium dynamics in astrocytes and neurons from adult mouse brain slices using single-photon GCaMP imaging
Confocal, multiphoton, or other advanced microscopy techniques produce high-quality datasets of calcium activity in live tissue. However, researchers without access to such expensive equipment can still produce meaningful observations from single-photon datasets. Here, we describe a protocol to extr...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Elsevier
2021
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7856475/ https://www.ncbi.nlm.nih.gov/pubmed/33554141 http://dx.doi.org/10.1016/j.xpro.2021.100306 |