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Intracolonic Mustard Oil Induces Visceral Pain in Mice by TRPA1-Dependent and -Independent Mechanisms: Role of Tissue Injury and P2X Receptors

Both TRPA1 and purinergic P2X receptors have been proposed as potential targets for the treatment of visceral pain. We found that the intracolonic administration of a low dose mustard oil (0.5%), a well-known TRPA1 agonist, produced nociceptive responses and abdominal wall referred mechanical hypera...

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Autores principales: Gonzalez-Cano, Rafael, Montilla-García, Ángeles, Perazzoli, Gloria, Torres, Jesús M., Cañizares, Francisco J., Fernández-Segura, Eduardo, Costigan, Michael, Baeyens, José M., Cobos, Enrique J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7859884/
https://www.ncbi.nlm.nih.gov/pubmed/33551815
http://dx.doi.org/10.3389/fphar.2020.613068
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author Gonzalez-Cano, Rafael
Montilla-García, Ángeles
Perazzoli, Gloria
Torres, Jesús M.
Cañizares, Francisco J.
Fernández-Segura, Eduardo
Costigan, Michael
Baeyens, José M.
Cobos, Enrique J.
author_facet Gonzalez-Cano, Rafael
Montilla-García, Ángeles
Perazzoli, Gloria
Torres, Jesús M.
Cañizares, Francisco J.
Fernández-Segura, Eduardo
Costigan, Michael
Baeyens, José M.
Cobos, Enrique J.
author_sort Gonzalez-Cano, Rafael
collection PubMed
description Both TRPA1 and purinergic P2X receptors have been proposed as potential targets for the treatment of visceral pain. We found that the intracolonic administration of a low dose mustard oil (0.5%), a well-known TRPA1 agonist, produced nociceptive responses and abdominal wall referred mechanical hyperalgesia, without inducing apparent tissue damage. Both nociceptive responses and referred hyperalgesia were abolished by the ablation of TRPV1-expressing neurons (and the consequent ablation of TRPA1+ nociceptors) by resiniferatoxin (RTX) treatment, and by the TRPA1 antagonist AP18. However, a higher dose of mustard oil (2.5%) damaged the colonic epithelium and induced pERK activation in the spinal cord, and these processes were clearly independent of TRPV1-expressing neurons ablated by RTX. This higher dose of mustard oil induced nociceptive responses and referred mechanical hyperalgesia which were insensitive or only slightly sensitive to resiniferatoxin or AP18, but were markedly reduced by the P2X antagonist TNP-ATP, which is known to inhibit nociceptive actions induced by ATP released from injured tissues. In conclusion, whereas a low dose of intracolonic mustard oil induces visceral pain in a manner fully dependent on TRPA1 actions, when a high dose of this chemical irritant is used, visceral pain becomes mostly independent of TRPA1 activation but clearly enhanced by ATP purportedly released by the damaged colonic epithelium. Therefore, TRPA1 inhibition is not sufficient to substantially decrease visceral pain during tissue injury, whereas purinergic antagonism appears to be a more effective strategy.
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spelling pubmed-78598842021-02-05 Intracolonic Mustard Oil Induces Visceral Pain in Mice by TRPA1-Dependent and -Independent Mechanisms: Role of Tissue Injury and P2X Receptors Gonzalez-Cano, Rafael Montilla-García, Ángeles Perazzoli, Gloria Torres, Jesús M. Cañizares, Francisco J. Fernández-Segura, Eduardo Costigan, Michael Baeyens, José M. Cobos, Enrique J. Front Pharmacol Pharmacology Both TRPA1 and purinergic P2X receptors have been proposed as potential targets for the treatment of visceral pain. We found that the intracolonic administration of a low dose mustard oil (0.5%), a well-known TRPA1 agonist, produced nociceptive responses and abdominal wall referred mechanical hyperalgesia, without inducing apparent tissue damage. Both nociceptive responses and referred hyperalgesia were abolished by the ablation of TRPV1-expressing neurons (and the consequent ablation of TRPA1+ nociceptors) by resiniferatoxin (RTX) treatment, and by the TRPA1 antagonist AP18. However, a higher dose of mustard oil (2.5%) damaged the colonic epithelium and induced pERK activation in the spinal cord, and these processes were clearly independent of TRPV1-expressing neurons ablated by RTX. This higher dose of mustard oil induced nociceptive responses and referred mechanical hyperalgesia which were insensitive or only slightly sensitive to resiniferatoxin or AP18, but were markedly reduced by the P2X antagonist TNP-ATP, which is known to inhibit nociceptive actions induced by ATP released from injured tissues. In conclusion, whereas a low dose of intracolonic mustard oil induces visceral pain in a manner fully dependent on TRPA1 actions, when a high dose of this chemical irritant is used, visceral pain becomes mostly independent of TRPA1 activation but clearly enhanced by ATP purportedly released by the damaged colonic epithelium. Therefore, TRPA1 inhibition is not sufficient to substantially decrease visceral pain during tissue injury, whereas purinergic antagonism appears to be a more effective strategy. Frontiers Media S.A. 2021-01-21 /pmc/articles/PMC7859884/ /pubmed/33551815 http://dx.doi.org/10.3389/fphar.2020.613068 Text en Copyright © 2021 Gonzalez-Cano, Montilla-García, Perazzoli, Torres, Cañizares, Fernãndez-Segura, Costigan, Baeyens and Cobos. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the >Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Gonzalez-Cano, Rafael
Montilla-García, Ángeles
Perazzoli, Gloria
Torres, Jesús M.
Cañizares, Francisco J.
Fernández-Segura, Eduardo
Costigan, Michael
Baeyens, José M.
Cobos, Enrique J.
Intracolonic Mustard Oil Induces Visceral Pain in Mice by TRPA1-Dependent and -Independent Mechanisms: Role of Tissue Injury and P2X Receptors
title Intracolonic Mustard Oil Induces Visceral Pain in Mice by TRPA1-Dependent and -Independent Mechanisms: Role of Tissue Injury and P2X Receptors
title_full Intracolonic Mustard Oil Induces Visceral Pain in Mice by TRPA1-Dependent and -Independent Mechanisms: Role of Tissue Injury and P2X Receptors
title_fullStr Intracolonic Mustard Oil Induces Visceral Pain in Mice by TRPA1-Dependent and -Independent Mechanisms: Role of Tissue Injury and P2X Receptors
title_full_unstemmed Intracolonic Mustard Oil Induces Visceral Pain in Mice by TRPA1-Dependent and -Independent Mechanisms: Role of Tissue Injury and P2X Receptors
title_short Intracolonic Mustard Oil Induces Visceral Pain in Mice by TRPA1-Dependent and -Independent Mechanisms: Role of Tissue Injury and P2X Receptors
title_sort intracolonic mustard oil induces visceral pain in mice by trpa1-dependent and -independent mechanisms: role of tissue injury and p2x receptors
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7859884/
https://www.ncbi.nlm.nih.gov/pubmed/33551815
http://dx.doi.org/10.3389/fphar.2020.613068
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