Targeted RNAseq assay incorporating unique molecular identifiers for improved quantification of gene expression signatures and transcribed mutation fraction in fixed tumor samples

BACKGROUND: Our objective was to assess whether modifications to a customized targeted RNA sequencing (RNAseq) assay to include unique molecular identifiers (UMIs) that collapse read counts to their source mRNA counts would improve quantification of transcripts from formalin-fixed paraffin-embedded...

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Detalles Bibliográficos
Autores principales: Fu, Chunxiao, Marczyk, Michal, Samuels, Michael, Trevarton, Alexander J., Qu, Jiaxin, Lau, Rosanna, Du, Lili, Pappas, Todd, Sinn, Bruno V., Gould, Rebekah E., Pusztai, Lajos, Hatzis, Christos, Symmans, W. Fraser
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Technical Advance
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7860187/
https://www.ncbi.nlm.nih.gov/pubmed/33541297
http://dx.doi.org/10.1186/s12885-021-07814-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7860187/
https://www.ncbi.nlm.nih.gov/pubmed/33541297
http://dx.doi.org/10.1186/s12885-021-07814-8

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