A multiplex CRISPR interference tool for virulence gene interrogation in Legionella pneumophila

Catalytically inactive dCas9 imposes transcriptional gene repression by sterically precluding RNA polymerase activity at a given gene to which it was directed by CRISPR (cr)RNAs. This gene silencing technology, known as CRISPR interference (CRISPRi), has been employed in various bacterial species to...

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Detalles Bibliográficos
Autores principales: Ellis, Nicole A., Kim, Byoungkwan, Tung, Jessica, Machner, Matthias P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7862264/
https://www.ncbi.nlm.nih.gov/pubmed/33542442
http://dx.doi.org/10.1038/s42003-021-01672-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7862264/
https://www.ncbi.nlm.nih.gov/pubmed/33542442
http://dx.doi.org/10.1038/s42003-021-01672-7

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