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Second-generation lung-on-a-chip with an array of stretchable alveoli made with a biological membrane

The air-blood barrier with its complex architecture and dynamic environment is difficult to mimic in vitro. Lung-on-a-chips enable mimicking the breathing movements using a thin, stretchable PDMS membrane. However, they fail to reproduce the characteristic alveoli network as well as the biochemical...

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Autores principales: Zamprogno, Pauline, Wüthrich, Simon, Achenbach, Sven, Thoma, Giuditta, Stucki, Janick D., Hobi, Nina, Schneider-Daum, Nicole, Lehr, Claus-Michael, Huwer, Hanno, Geiser, Thomas, Schmid, Ralph A., Guenat, Olivier T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7864995/
https://www.ncbi.nlm.nih.gov/pubmed/33547387
http://dx.doi.org/10.1038/s42003-021-01695-0
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author Zamprogno, Pauline
Wüthrich, Simon
Achenbach, Sven
Thoma, Giuditta
Stucki, Janick D.
Hobi, Nina
Schneider-Daum, Nicole
Lehr, Claus-Michael
Huwer, Hanno
Geiser, Thomas
Schmid, Ralph A.
Guenat, Olivier T.
author_facet Zamprogno, Pauline
Wüthrich, Simon
Achenbach, Sven
Thoma, Giuditta
Stucki, Janick D.
Hobi, Nina
Schneider-Daum, Nicole
Lehr, Claus-Michael
Huwer, Hanno
Geiser, Thomas
Schmid, Ralph A.
Guenat, Olivier T.
author_sort Zamprogno, Pauline
collection PubMed
description The air-blood barrier with its complex architecture and dynamic environment is difficult to mimic in vitro. Lung-on-a-chips enable mimicking the breathing movements using a thin, stretchable PDMS membrane. However, they fail to reproduce the characteristic alveoli network as well as the biochemical and physical properties of the alveolar basal membrane. Here, we present a lung-on-a-chip, based on a biological, stretchable and biodegradable membrane made of collagen and elastin, that emulates an array of tiny alveoli with in vivo-like dimensions. This membrane outperforms PDMS in many ways: it does not absorb rhodamine-B, is biodegradable, is created by a simple method, and can easily be tuned to modify its thickness, composition and stiffness. The air-blood barrier is reconstituted using primary lung alveolar epithelial cells from patients and primary lung endothelial cells. Typical alveolar epithelial cell markers are expressed, while the barrier properties are preserved for up to 3 weeks.
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spelling pubmed-78649952021-02-16 Second-generation lung-on-a-chip with an array of stretchable alveoli made with a biological membrane Zamprogno, Pauline Wüthrich, Simon Achenbach, Sven Thoma, Giuditta Stucki, Janick D. Hobi, Nina Schneider-Daum, Nicole Lehr, Claus-Michael Huwer, Hanno Geiser, Thomas Schmid, Ralph A. Guenat, Olivier T. Commun Biol Article The air-blood barrier with its complex architecture and dynamic environment is difficult to mimic in vitro. Lung-on-a-chips enable mimicking the breathing movements using a thin, stretchable PDMS membrane. However, they fail to reproduce the characteristic alveoli network as well as the biochemical and physical properties of the alveolar basal membrane. Here, we present a lung-on-a-chip, based on a biological, stretchable and biodegradable membrane made of collagen and elastin, that emulates an array of tiny alveoli with in vivo-like dimensions. This membrane outperforms PDMS in many ways: it does not absorb rhodamine-B, is biodegradable, is created by a simple method, and can easily be tuned to modify its thickness, composition and stiffness. The air-blood barrier is reconstituted using primary lung alveolar epithelial cells from patients and primary lung endothelial cells. Typical alveolar epithelial cell markers are expressed, while the barrier properties are preserved for up to 3 weeks. Nature Publishing Group UK 2021-02-05 /pmc/articles/PMC7864995/ /pubmed/33547387 http://dx.doi.org/10.1038/s42003-021-01695-0 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zamprogno, Pauline
Wüthrich, Simon
Achenbach, Sven
Thoma, Giuditta
Stucki, Janick D.
Hobi, Nina
Schneider-Daum, Nicole
Lehr, Claus-Michael
Huwer, Hanno
Geiser, Thomas
Schmid, Ralph A.
Guenat, Olivier T.
Second-generation lung-on-a-chip with an array of stretchable alveoli made with a biological membrane
title Second-generation lung-on-a-chip with an array of stretchable alveoli made with a biological membrane
title_full Second-generation lung-on-a-chip with an array of stretchable alveoli made with a biological membrane
title_fullStr Second-generation lung-on-a-chip with an array of stretchable alveoli made with a biological membrane
title_full_unstemmed Second-generation lung-on-a-chip with an array of stretchable alveoli made with a biological membrane
title_short Second-generation lung-on-a-chip with an array of stretchable alveoli made with a biological membrane
title_sort second-generation lung-on-a-chip with an array of stretchable alveoli made with a biological membrane
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7864995/
https://www.ncbi.nlm.nih.gov/pubmed/33547387
http://dx.doi.org/10.1038/s42003-021-01695-0
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