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Influence of germinated brown rice‐based flour modified by MAse on type 2 diabetic mice and HepG2 cell cytotoxic capacity
This study aimed to discover whether using maltogenic amylase (MAse) to modify starch in germinated brown rice flour may enhance slow digestion starch and release more bioactive compounds (BCs) content. To achieve this aim, the starch was modified with four levels of MAse (0 U, 133 U, 266 U and 399...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7866618/ https://www.ncbi.nlm.nih.gov/pubmed/33598163 http://dx.doi.org/10.1002/fsn3.2043 |
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author | Nguyen, Ngoc Thi Le Nguyen, Binh Duong Thanh Dai, Trang Thi Xuan Co, Son Hong Do, Thao Thi Tong Thi, Anh Ngoc Oladapo, Ibitoye Joshua Nguyen Cong, Ha |
author_facet | Nguyen, Ngoc Thi Le Nguyen, Binh Duong Thanh Dai, Trang Thi Xuan Co, Son Hong Do, Thao Thi Tong Thi, Anh Ngoc Oladapo, Ibitoye Joshua Nguyen Cong, Ha |
author_sort | Nguyen, Ngoc Thi Le |
collection | PubMed |
description | This study aimed to discover whether using maltogenic amylase (MAse) to modify starch in germinated brown rice flour may enhance slow digestion starch and release more bioactive compounds (BCs) content. To achieve this aim, the starch was modified with four levels of MAse (0 U, 133 U, 266 U and 399 U MAse/g flour) for 1 hr at pH 5 and then spray‐dried to make modified flour. The biochemical impacts of the products were then accessed in normal and type 2 diabetic mice for 4 weeks. The result showed that when the starch was modified by MAse 266 U/g, a significant reduction of rapidly digested starch to 22.35% from 61.56%, an increase in slowly digested starch to 33.09% while resistant starch as 2.92% corresponding to the increase of γ‐amino butyric acid to 528.1 ± 44.1 mg/L and 120.6 ± 10.9 mg/L of ferulic acid. The extract from modified flour showed very strong cytotoxic activity against HepG2 cell (>80% inhibition). The result in vivo showed that the type‐2 diabetic mice fed with this modified product could better improve the stability of the glycemic index. Also, atherosclerotic plaque assessment further supports these findings. The results indicated that BCs released considerably couple with the changes in starch properties caused by MAse enhanced the effectiveness of this product to diabetes as well as positive effect on cytotoxic activity against HepG2 cell. |
format | Online Article Text |
id | pubmed-7866618 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-78666182021-02-16 Influence of germinated brown rice‐based flour modified by MAse on type 2 diabetic mice and HepG2 cell cytotoxic capacity Nguyen, Ngoc Thi Le Nguyen, Binh Duong Thanh Dai, Trang Thi Xuan Co, Son Hong Do, Thao Thi Tong Thi, Anh Ngoc Oladapo, Ibitoye Joshua Nguyen Cong, Ha Food Sci Nutr Original Research This study aimed to discover whether using maltogenic amylase (MAse) to modify starch in germinated brown rice flour may enhance slow digestion starch and release more bioactive compounds (BCs) content. To achieve this aim, the starch was modified with four levels of MAse (0 U, 133 U, 266 U and 399 U MAse/g flour) for 1 hr at pH 5 and then spray‐dried to make modified flour. The biochemical impacts of the products were then accessed in normal and type 2 diabetic mice for 4 weeks. The result showed that when the starch was modified by MAse 266 U/g, a significant reduction of rapidly digested starch to 22.35% from 61.56%, an increase in slowly digested starch to 33.09% while resistant starch as 2.92% corresponding to the increase of γ‐amino butyric acid to 528.1 ± 44.1 mg/L and 120.6 ± 10.9 mg/L of ferulic acid. The extract from modified flour showed very strong cytotoxic activity against HepG2 cell (>80% inhibition). The result in vivo showed that the type‐2 diabetic mice fed with this modified product could better improve the stability of the glycemic index. Also, atherosclerotic plaque assessment further supports these findings. The results indicated that BCs released considerably couple with the changes in starch properties caused by MAse enhanced the effectiveness of this product to diabetes as well as positive effect on cytotoxic activity against HepG2 cell. John Wiley and Sons Inc. 2020-11-29 /pmc/articles/PMC7866618/ /pubmed/33598163 http://dx.doi.org/10.1002/fsn3.2043 Text en © 2020 The Authors. Food Science & Nutrition published by Wiley Periodicals LLC This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Nguyen, Ngoc Thi Le Nguyen, Binh Duong Thanh Dai, Trang Thi Xuan Co, Son Hong Do, Thao Thi Tong Thi, Anh Ngoc Oladapo, Ibitoye Joshua Nguyen Cong, Ha Influence of germinated brown rice‐based flour modified by MAse on type 2 diabetic mice and HepG2 cell cytotoxic capacity |
title | Influence of germinated brown rice‐based flour modified by MAse on type 2 diabetic mice and HepG2 cell cytotoxic capacity |
title_full | Influence of germinated brown rice‐based flour modified by MAse on type 2 diabetic mice and HepG2 cell cytotoxic capacity |
title_fullStr | Influence of germinated brown rice‐based flour modified by MAse on type 2 diabetic mice and HepG2 cell cytotoxic capacity |
title_full_unstemmed | Influence of germinated brown rice‐based flour modified by MAse on type 2 diabetic mice and HepG2 cell cytotoxic capacity |
title_short | Influence of germinated brown rice‐based flour modified by MAse on type 2 diabetic mice and HepG2 cell cytotoxic capacity |
title_sort | influence of germinated brown rice‐based flour modified by mase on type 2 diabetic mice and hepg2 cell cytotoxic capacity |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7866618/ https://www.ncbi.nlm.nih.gov/pubmed/33598163 http://dx.doi.org/10.1002/fsn3.2043 |
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