Genome-scale chemogenomic CRISPR screens in human cells using the TKOv3 library

CRISPR-based genetic screens revolutionized our ability to genetically probe cell biology. We present a protocol to conduct genome-scale chemogenomic dropout CRISPR screens in the human RPE1-hTERT p53(−/−) cell line. We use the TKOv3 library, which contains 70,948 sgRNAs targeting 18,053 genes. Here...

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Detalles Bibliográficos
Autores principales: Olivieri, Michele, Durocher, Daniel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7868615/
https://www.ncbi.nlm.nih.gov/pubmed/33598657
http://dx.doi.org/10.1016/j.xpro.2021.100321

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7868615/
https://www.ncbi.nlm.nih.gov/pubmed/33598657
http://dx.doi.org/10.1016/j.xpro.2021.100321

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