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Genome-scale chemogenomic CRISPR screens in human cells using the TKOv3 library
CRISPR-based genetic screens revolutionized our ability to genetically probe cell biology. We present a protocol to conduct genome-scale chemogenomic dropout CRISPR screens in the human RPE1-hTERT p53(−/−) cell line. We use the TKOv3 library, which contains 70,948 sgRNAs targeting 18,053 genes. Here...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7868615/ https://www.ncbi.nlm.nih.gov/pubmed/33598657 http://dx.doi.org/10.1016/j.xpro.2021.100321 |