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Senescence associated long non-coding RNA 1 regulates cigarette smoke-induced senescence of type II alveolar epithelial cells through sirtuin-1 signaling
OBJECTIVE: The primary aim of our study was to explore the mechanisms through which long non-coding RNA (lncRNA)-mediated sirtuin-1 (SIRT1) signaling regulates type II alveolar epithelial cell (AECII) senescence induced by a cigarette smoke-media suspension (CSM). METHODS: Pharmacological SIRT1 acti...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7869169/ https://www.ncbi.nlm.nih.gov/pubmed/33535826 http://dx.doi.org/10.1177/0300060520986049 |
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author | Yuan, Dong Liu, Yuanshun Li, Mengyu Zhou, Hongbin Cao, Liming Zhang, Xiaoqin Li, Yaqing |
author_facet | Yuan, Dong Liu, Yuanshun Li, Mengyu Zhou, Hongbin Cao, Liming Zhang, Xiaoqin Li, Yaqing |
author_sort | Yuan, Dong |
collection | PubMed |
description | OBJECTIVE: The primary aim of our study was to explore the mechanisms through which long non-coding RNA (lncRNA)-mediated sirtuin-1 (SIRT1) signaling regulates type II alveolar epithelial cell (AECII) senescence induced by a cigarette smoke-media suspension (CSM). METHODS: Pharmacological SIRT1 activation was induced using SRT2104 and senescence-associated lncRNA 1 (SAL-RNA1) was overexpressed. The expression of SIRT1, FOXO3a, p53, p21, MMP-9, and TIMP-1 in different groups was detected by qRT-PCR and Western blotting; the activity of SA-β gal was detected by staining; the binding of SIRT1 to FOXO3a and p53 gene transcription promoters was detected by Chip. RESULTS: We found that CSM increased AECII senescence, while SAL-RNA1 overexpression and SIRT1 activation significantly decreased levels of AECII senescence induced by CSM. Using chromatin immunoprecipitation, we found that SIRT1 bound differentially to transcriptional complexes on the FOXO3a and p53 promoters. CONCLUSION: Our results suggested that lncRNA-SAL1-mediated SIRT1 signaling reduces senescence of AECIIs induced by CSM. These findings suggest a new therapeutic target to limit the irreversible apoptosis of lung epithelial cells in COPD patients. |
format | Online Article Text |
id | pubmed-7869169 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-78691692021-02-19 Senescence associated long non-coding RNA 1 regulates cigarette smoke-induced senescence of type II alveolar epithelial cells through sirtuin-1 signaling Yuan, Dong Liu, Yuanshun Li, Mengyu Zhou, Hongbin Cao, Liming Zhang, Xiaoqin Li, Yaqing J Int Med Res Pre-Clinical Research Report OBJECTIVE: The primary aim of our study was to explore the mechanisms through which long non-coding RNA (lncRNA)-mediated sirtuin-1 (SIRT1) signaling regulates type II alveolar epithelial cell (AECII) senescence induced by a cigarette smoke-media suspension (CSM). METHODS: Pharmacological SIRT1 activation was induced using SRT2104 and senescence-associated lncRNA 1 (SAL-RNA1) was overexpressed. The expression of SIRT1, FOXO3a, p53, p21, MMP-9, and TIMP-1 in different groups was detected by qRT-PCR and Western blotting; the activity of SA-β gal was detected by staining; the binding of SIRT1 to FOXO3a and p53 gene transcription promoters was detected by Chip. RESULTS: We found that CSM increased AECII senescence, while SAL-RNA1 overexpression and SIRT1 activation significantly decreased levels of AECII senescence induced by CSM. Using chromatin immunoprecipitation, we found that SIRT1 bound differentially to transcriptional complexes on the FOXO3a and p53 promoters. CONCLUSION: Our results suggested that lncRNA-SAL1-mediated SIRT1 signaling reduces senescence of AECIIs induced by CSM. These findings suggest a new therapeutic target to limit the irreversible apoptosis of lung epithelial cells in COPD patients. SAGE Publications 2021-02-04 /pmc/articles/PMC7869169/ /pubmed/33535826 http://dx.doi.org/10.1177/0300060520986049 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by-nc/4.0/ Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Pre-Clinical Research Report Yuan, Dong Liu, Yuanshun Li, Mengyu Zhou, Hongbin Cao, Liming Zhang, Xiaoqin Li, Yaqing Senescence associated long non-coding RNA 1 regulates cigarette smoke-induced senescence of type II alveolar epithelial cells through sirtuin-1 signaling |
title | Senescence associated long non-coding RNA 1 regulates cigarette
smoke-induced senescence of type II alveolar epithelial cells through sirtuin-1
signaling |
title_full | Senescence associated long non-coding RNA 1 regulates cigarette
smoke-induced senescence of type II alveolar epithelial cells through sirtuin-1
signaling |
title_fullStr | Senescence associated long non-coding RNA 1 regulates cigarette
smoke-induced senescence of type II alveolar epithelial cells through sirtuin-1
signaling |
title_full_unstemmed | Senescence associated long non-coding RNA 1 regulates cigarette
smoke-induced senescence of type II alveolar epithelial cells through sirtuin-1
signaling |
title_short | Senescence associated long non-coding RNA 1 regulates cigarette
smoke-induced senescence of type II alveolar epithelial cells through sirtuin-1
signaling |
title_sort | senescence associated long non-coding rna 1 regulates cigarette
smoke-induced senescence of type ii alveolar epithelial cells through sirtuin-1
signaling |
topic | Pre-Clinical Research Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7869169/ https://www.ncbi.nlm.nih.gov/pubmed/33535826 http://dx.doi.org/10.1177/0300060520986049 |
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