Cargando…

A Semi-automated Organoid Screening Method Demonstrates Epigenetic Control of Intestinal Epithelial Differentiation

Intestinal organoids are an excellent model to study epithelial biology. Yet, the selection of analytical tools to accurately quantify heterogeneous organoid cultures remains limited. Here, we developed a semi-automated organoid screening method, which we applied to a library of highly specific chem...

Descripción completa

Detalles Bibliográficos
Autores principales:	Ostrop, Jenny, Zwiggelaar, Rosalie T., Terndrup Pedersen, Marianne, Gerbe, François, Bösl, Korbinian, Lindholm, Håvard T., Díez-Sánchez, Alberto, Parmar, Naveen, Radetzki, Silke, von Kries, Jens Peter, Jay, Philippe, Jensen, Kim B., Arrowsmith, Cheryl, Oudhoff, Menno J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2021
Materias:	Cell and Developmental Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7872100/ https://www.ncbi.nlm.nih.gov/pubmed/33575256 http://dx.doi.org/10.3389/fcell.2020.618552

Ejemplares similares

LSD1 represses a neonatal/reparative gene program in adult intestinal epithelium
por: Zwiggelaar, Rosalie T., et al.
Publicado: (2020)

BMP-signaling in the intestinal epithelium drives a critical feedback loop to restrain IL-13-driven tuft cell hyperplasia
por: Lindholm, Håvard T., et al.
Publicado: (2022)

Intestinal-epithelial LSD1 controls goblet cell maturation and effector responses required for gut immunity to bacterial and helminth infection
por: Parmar, Naveen, et al.
Publicado: (2021)

Intestinal epithelial tuft cell induction is negated by a murine helminth and its secreted products
por: Drurey, Claire, et al.
Publicado: (2021)

Mmp17-deficient mice exhibit heightened goblet cell effector expression in the colon and increased resistance to chronic Trichuris muris infection
por: Vornewald, Pia M., et al.
Publicado: (2023)

Smooth muscle-specific MMP17 (MT4-MMP) regulates the intestinal stem cell niche and regeneration after damage
por: Martín-Alonso, Mara, et al.
Publicado: (2021)

Organoids demonstrate gut infection by SARS-CoV-2
por: Dickson, Iain
Publicado: (2020)

Organoid technology demonstrates effects of potential drugs for COVID‐19 on the lung regeneration
por: Wang, Jianhai, et al.
Publicado: (2020)

High-Throughput Screening for Modulators of CFTR Activity Based on Genetically Engineered Cystic Fibrosis Disease-Specific iPSCs
por: Merkert, Sylvia, et al.
Publicado: (2019)

Novel human pluripotent stem cell-derived hypothalamus organoids demonstrate cellular diversity
por: Sarrafha, Lily, et al.
Publicado: (2023)

Experimental demonstration of an efficient, semi-device-independent photonic indistinguishability witness
por: van der Meer, Reinier, et al.
Publicado: (2021)

Topical inflammasome inhibition with disulfiram prevents irritant contact dermatitis
por: Bonnekoh, Hanna, et al.
Publicado: (2021)

Semi-Automated Computational Assessment of Cancer Organoid Viability Using Rapid Live-Cell Microscopy
por: Buehler, Joseph D, et al.
Publicado: (2022)

Statin and rottlerin small-molecule inhibitors restrict colon cancer progression and metastasis via MACC1
por: Juneja, Manisha, et al.
Publicado: (2017)

TB-2 Patient-derived meningioma organoid model demonstrates FOXM1 dependent tumor proliferation
por: Yamazaki, Shintaro, et al.
Publicado: (2021)

Matrix scaffolds for endometrium-derived organoid models
por: De Vriendt, Silke, et al.
Publicado: (2023)

Opioid Considerations for Emergency Practice
por: Terndrup, Thomas
Publicado: (2015)

Revealing cytotoxic substructures in molecules using deep learning
por: Webel, Henry E., et al.
Publicado: (2020)

Dynamics of Dual Specificity Phosphatases and Their Interplay with Protein Kinases in Immune Signaling
por: Subbannayya, Yashwanth, et al.
Publicado: (2019)

Surgical teaching in urology: patient safety and educational value of ‘LIVE’ and ‘SEMI-LIVE’ surgical demonstrations
por: Legemate, Jaap D., et al.
Publicado: (2018)

Autism in Three Dimensions: Using Brain Organoids to Study Potential Gene–Environment Interactions
por: Schmidt, Silke
Publicado: (2021)

Comparative Study on Local Binary Patterns for Mammographic Density and Risk Scoring †
por: George, Minu, et al.
Publicado: (2019)

Review on the Vascularization of Organoids and Organoids-on-a-Chip
por: Zhao, Xingli, et al.
Publicado: (2021)

RNA-sequencing of single cholangiocyte-derived organoids reveals high organoid-to organoid variability
por: Gehling, Kristin, et al.
Publicado: (2022)

Semi-Automated Recording of Facial Sensitivity in Rat Demonstrates Antinociceptive Effects of the Anti-CGRP Antibody Fremanezumab
por: Benedicter, Nicola, et al.
Publicado: (2023)

Self-Assembled Matrigel-Free iPSC-Derived Liver Organoids Demonstrate Wide-Ranging Highly Differentiated Liver Functions
por: Weng, Yun, et al.
Publicado: (2022)

Organoids are not organs: Sources of variation and misinformation in organoid biology
por: Jensen, Kim Bak, et al.
Publicado: (2023)

Intestinal Epithelial Cell-Intrinsic Deletion of Setd7 Identifies Role for Developmental Pathways in Immunity to Helminth Infection
por: Oudhoff, Menno J., et al.
Publicado: (2016)

Patient-Derived Organoids in Precision Medicine: Drug Screening, Organoid-on-a-Chip and Living Organoid Biobank
por: Zhou, Zilong, et al.
Publicado: (2021)

Semi-permanent smartphone adapter for microscopes: Design demonstration and workflow testing using a slit-lamp biomicroscope
por: Yen, Chieh-Hung, et al.
Publicado: (2019)

Myofibre segmentation in H&E stained adult skeletal muscle images using coherence-enhancing diffusion filtering
por: Strange, Harry, et al.
Publicado: (2014)

Coactivation of TLR2 and TLR8 in Primary Human Monocytes Triggers a Distinct Inflammatory Signaling Response
por: Bösl, Korbinian, et al.
Publicado: (2018)

Engineering organoids
por: Hofer, Moritz, et al.
Publicado: (2021)

MammaPrint versus EndoPredict: Poor correlation in disease recurrence risk classification of hormone receptor positive breast cancer
por: Bösl, Andreas, et al.
Publicado: (2017)

The intestinal epithelium tuft cells: specification and function
por: Gerbe, François, et al.
Publicado: (2012)

Linked Color Imaging Demonstrates Characteristic Findings in Semi-Pedunculated Gastric Adenocarcinoma in Helicobacter pylori-Negative Normal Mucosa
por: Hiraoka, Yuji, et al.
Publicado: (2021)

Research proposal content extraction using natural language processing and semi-supervised clustering: A demonstration and comparative analysis
por: Knisely, Benjamin M., et al.
Publicado: (2023)

A semi-automated, KNIME-based workflow for biofilm assays
por: Leinweber, Katrin, et al.
Publicado: (2016)

The Effect of Compressor-administered Defibrillation on Peri-shock Pauses in a Simulated Cardiac Arrest Scenario
por: Glick, Joshua, et al.
Publicado: (2014)

OrganoidDB: a comprehensive organoid database for the multi-perspective exploration of bulk and single-cell transcriptomic profiles of organoids
por: Ma, Qinfeng, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_ov4odpoqqlrrc4b40mhtuksst6