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Rapid profiling of drug-resistant bacteria using DNA-binding dyes and a nanopore-based DNA sequencer

Spread of drug-resistant bacteria is a serious problem worldwide. We thus designed a new sequence-based protocol that can quickly identify bacterial compositions of clinical samples and their drug-resistance profiles simultaneously. Here we utilized propidium monoazide (PMA) that prohibits DNA ampli...

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Detalles Bibliográficos
Autores principales: Ohno, Ayumu, Umezawa, Kazuo, Asai, Satomi, Kryukov, Kirill, Nakagawa, So, Miyachi, Hayato, Imanishi, Tadashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7873225/
https://www.ncbi.nlm.nih.gov/pubmed/33564026
http://dx.doi.org/10.1038/s41598-021-82903-z