Cargando…

Emerging Technologies for Genome-Wide Profiling of DNA Breakage

Genome instability is associated with myriad human diseases and is a well-known feature of both cancer and neurodegenerative disease. Until recently, the ability to assess DNA damage—the principal driver of genome instability—was limited to relatively imprecise methods or restricted to studying pred...

Descripción completa

Detalles Bibliográficos
Autores principales: Rybin, Matthew J., Ramic, Melina, Ricciardi, Natalie R., Kapranov, Philipp, Wahlestedt, Claes, Zeier, Zane
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7873462/
https://www.ncbi.nlm.nih.gov/pubmed/33584810
http://dx.doi.org/10.3389/fgene.2020.610386
_version_ 1783649390604320768
author Rybin, Matthew J.
Ramic, Melina
Ricciardi, Natalie R.
Kapranov, Philipp
Wahlestedt, Claes
Zeier, Zane
author_facet Rybin, Matthew J.
Ramic, Melina
Ricciardi, Natalie R.
Kapranov, Philipp
Wahlestedt, Claes
Zeier, Zane
author_sort Rybin, Matthew J.
collection PubMed
description Genome instability is associated with myriad human diseases and is a well-known feature of both cancer and neurodegenerative disease. Until recently, the ability to assess DNA damage—the principal driver of genome instability—was limited to relatively imprecise methods or restricted to studying predefined genomic regions. Recently, new techniques for detecting DNA double strand breaks (DSBs) and single strand breaks (SSBs) with next-generation sequencing on a genome-wide scale with single nucleotide resolution have emerged. With these new tools, efforts are underway to define the “breakome” in normal aging and disease. Here, we compare the relative strengths and weaknesses of these technologies and their potential application to studying neurodegenerative diseases.
format Online
Article
Text
id pubmed-7873462
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-78734622021-02-11 Emerging Technologies for Genome-Wide Profiling of DNA Breakage Rybin, Matthew J. Ramic, Melina Ricciardi, Natalie R. Kapranov, Philipp Wahlestedt, Claes Zeier, Zane Front Genet Genetics Genome instability is associated with myriad human diseases and is a well-known feature of both cancer and neurodegenerative disease. Until recently, the ability to assess DNA damage—the principal driver of genome instability—was limited to relatively imprecise methods or restricted to studying predefined genomic regions. Recently, new techniques for detecting DNA double strand breaks (DSBs) and single strand breaks (SSBs) with next-generation sequencing on a genome-wide scale with single nucleotide resolution have emerged. With these new tools, efforts are underway to define the “breakome” in normal aging and disease. Here, we compare the relative strengths and weaknesses of these technologies and their potential application to studying neurodegenerative diseases. Frontiers Media S.A. 2021-01-27 /pmc/articles/PMC7873462/ /pubmed/33584810 http://dx.doi.org/10.3389/fgene.2020.610386 Text en Copyright © 2021 Rybin, Ramic, Ricciardi, Kapranov, Wahlestedt and Zeier. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Rybin, Matthew J.
Ramic, Melina
Ricciardi, Natalie R.
Kapranov, Philipp
Wahlestedt, Claes
Zeier, Zane
Emerging Technologies for Genome-Wide Profiling of DNA Breakage
title Emerging Technologies for Genome-Wide Profiling of DNA Breakage
title_full Emerging Technologies for Genome-Wide Profiling of DNA Breakage
title_fullStr Emerging Technologies for Genome-Wide Profiling of DNA Breakage
title_full_unstemmed Emerging Technologies for Genome-Wide Profiling of DNA Breakage
title_short Emerging Technologies for Genome-Wide Profiling of DNA Breakage
title_sort emerging technologies for genome-wide profiling of dna breakage
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7873462/
https://www.ncbi.nlm.nih.gov/pubmed/33584810
http://dx.doi.org/10.3389/fgene.2020.610386
work_keys_str_mv AT rybinmatthewj emergingtechnologiesforgenomewideprofilingofdnabreakage
AT ramicmelina emergingtechnologiesforgenomewideprofilingofdnabreakage
AT ricciardinatalier emergingtechnologiesforgenomewideprofilingofdnabreakage
AT kapranovphilipp emergingtechnologiesforgenomewideprofilingofdnabreakage
AT wahlestedtclaes emergingtechnologiesforgenomewideprofilingofdnabreakage
AT zeierzane emergingtechnologiesforgenomewideprofilingofdnabreakage