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Protocol for dissection and culture of murine dorsal root ganglia neurons to study neuropeptide release

In this protocol, we provide step-by-step instructions for dissection and culture of primary murine dorsal root ganglia (DRG), which provide an opportunity to study the functional properties of peripheral sensory neurons in vitro. Further, we describe the analysis of neuropeptide release by ELISA as...

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Detalles Bibliográficos
Autores principales: Perner, Caroline, Sokol, Caroline L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7876630/
https://www.ncbi.nlm.nih.gov/pubmed/33615276
http://dx.doi.org/10.1016/j.xpro.2021.100333