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Tsetse salivary glycoproteins are modified with paucimannosidic N-glycans, are recognised by C-type lectins and bind to trypanosomes
African sleeping sickness is caused by Trypanosoma brucei, a parasite transmitted by the bite of a tsetse fly. Trypanosome infection induces a severe transcriptional downregulation of tsetse genes encoding for salivary proteins, which reduces its anti-hemostatic and anti-clotting properties. To bett...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7880456/ https://www.ncbi.nlm.nih.gov/pubmed/33529215 http://dx.doi.org/10.1371/journal.pntd.0009071 |
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author | Kozak, Radoslaw P. Mondragon-Shem, Karina Williams, Christopher Rose, Clair Perally, Samirah Caljon, Guy Van Den Abbeele, Jan Wongtrakul-Kish, Katherine Gardner, Richard A. Spencer, Daniel Lehane, Michael J. Acosta-Serrano, Álvaro |
author_facet | Kozak, Radoslaw P. Mondragon-Shem, Karina Williams, Christopher Rose, Clair Perally, Samirah Caljon, Guy Van Den Abbeele, Jan Wongtrakul-Kish, Katherine Gardner, Richard A. Spencer, Daniel Lehane, Michael J. Acosta-Serrano, Álvaro |
author_sort | Kozak, Radoslaw P. |
collection | PubMed |
description | African sleeping sickness is caused by Trypanosoma brucei, a parasite transmitted by the bite of a tsetse fly. Trypanosome infection induces a severe transcriptional downregulation of tsetse genes encoding for salivary proteins, which reduces its anti-hemostatic and anti-clotting properties. To better understand trypanosome transmission and the possible role of glycans in insect bloodfeeding, we characterized the N-glycome of tsetse saliva glycoproteins. Tsetse salivary N-glycans were enzymatically released, tagged with either 2-aminobenzamide (2-AB) or procainamide, and analyzed by HILIC-UHPLC-FLR coupled online with positive-ion ESI-LC-MS/MS. We found that the N-glycan profiles of T. brucei-infected and naïve tsetse salivary glycoproteins are almost identical, consisting mainly (>50%) of highly processed Man(3)GlcNAc(2) in addition to several other paucimannose, high mannose, and few hybrid-type N-glycans. In overlay assays, these sugars were differentially recognized by the mannose receptor and DC-SIGN C-type lectins. We also show that salivary glycoproteins bind strongly to the surface of transmissible metacyclic trypanosomes. We suggest that although the repertoire of tsetse salivary N-glycans does not change during a trypanosome infection, the interactions with mannosylated glycoproteins may influence parasite transmission into the vertebrate host. |
format | Online Article Text |
id | pubmed-7880456 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-78804562021-02-19 Tsetse salivary glycoproteins are modified with paucimannosidic N-glycans, are recognised by C-type lectins and bind to trypanosomes Kozak, Radoslaw P. Mondragon-Shem, Karina Williams, Christopher Rose, Clair Perally, Samirah Caljon, Guy Van Den Abbeele, Jan Wongtrakul-Kish, Katherine Gardner, Richard A. Spencer, Daniel Lehane, Michael J. Acosta-Serrano, Álvaro PLoS Negl Trop Dis Research Article African sleeping sickness is caused by Trypanosoma brucei, a parasite transmitted by the bite of a tsetse fly. Trypanosome infection induces a severe transcriptional downregulation of tsetse genes encoding for salivary proteins, which reduces its anti-hemostatic and anti-clotting properties. To better understand trypanosome transmission and the possible role of glycans in insect bloodfeeding, we characterized the N-glycome of tsetse saliva glycoproteins. Tsetse salivary N-glycans were enzymatically released, tagged with either 2-aminobenzamide (2-AB) or procainamide, and analyzed by HILIC-UHPLC-FLR coupled online with positive-ion ESI-LC-MS/MS. We found that the N-glycan profiles of T. brucei-infected and naïve tsetse salivary glycoproteins are almost identical, consisting mainly (>50%) of highly processed Man(3)GlcNAc(2) in addition to several other paucimannose, high mannose, and few hybrid-type N-glycans. In overlay assays, these sugars were differentially recognized by the mannose receptor and DC-SIGN C-type lectins. We also show that salivary glycoproteins bind strongly to the surface of transmissible metacyclic trypanosomes. We suggest that although the repertoire of tsetse salivary N-glycans does not change during a trypanosome infection, the interactions with mannosylated glycoproteins may influence parasite transmission into the vertebrate host. Public Library of Science 2021-02-02 /pmc/articles/PMC7880456/ /pubmed/33529215 http://dx.doi.org/10.1371/journal.pntd.0009071 Text en © 2021 Kozak et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Kozak, Radoslaw P. Mondragon-Shem, Karina Williams, Christopher Rose, Clair Perally, Samirah Caljon, Guy Van Den Abbeele, Jan Wongtrakul-Kish, Katherine Gardner, Richard A. Spencer, Daniel Lehane, Michael J. Acosta-Serrano, Álvaro Tsetse salivary glycoproteins are modified with paucimannosidic N-glycans, are recognised by C-type lectins and bind to trypanosomes |
title | Tsetse salivary glycoproteins are modified with paucimannosidic N-glycans, are recognised by C-type lectins and bind to trypanosomes |
title_full | Tsetse salivary glycoproteins are modified with paucimannosidic N-glycans, are recognised by C-type lectins and bind to trypanosomes |
title_fullStr | Tsetse salivary glycoproteins are modified with paucimannosidic N-glycans, are recognised by C-type lectins and bind to trypanosomes |
title_full_unstemmed | Tsetse salivary glycoproteins are modified with paucimannosidic N-glycans, are recognised by C-type lectins and bind to trypanosomes |
title_short | Tsetse salivary glycoproteins are modified with paucimannosidic N-glycans, are recognised by C-type lectins and bind to trypanosomes |
title_sort | tsetse salivary glycoproteins are modified with paucimannosidic n-glycans, are recognised by c-type lectins and bind to trypanosomes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7880456/ https://www.ncbi.nlm.nih.gov/pubmed/33529215 http://dx.doi.org/10.1371/journal.pntd.0009071 |
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