Optimized gene expression from bacterial chromosome by high-throughput integration and screening

Chromosomal integration of recombinant genes is desirable compared with expression from plasmids due to increased stability, reduced cell-to-cell variability, and elimination of the need for antibiotics for plasmid maintenance. Here, we present a new approach for tuning pathway gene expression level...

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Detalles Bibliográficos
Autores principales: Saleski, Tatyana E., Chung, Meng Ting, Carruthers, David N., Khasbaatar, Azzaya, Kurabayashi, Katsuo, Lin, Xiaoxia Nina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2021
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7880599/
https://www.ncbi.nlm.nih.gov/pubmed/33579713
http://dx.doi.org/10.1126/sciadv.abe1767

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7880599/
https://www.ncbi.nlm.nih.gov/pubmed/33579713
http://dx.doi.org/10.1126/sciadv.abe1767

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