Generation of inner ear sensory neurons using blastocyst complementation in a Neurog1(+/−)−deficient mouse

This research is the first to produce induced pluripotent stem cell-derived inner ear sensory neurons in the Neurog1(+/−) heterozygote mouse using blastocyst complementation. Additionally, this approach corrected non-sensory deficits associated with Neurog1 heterozygosity, indicating that complement...

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Autores principales: Steevens, Aleta R., Griesbach, Matthew W., You, Yun, Dutton, James R., Low, Walter C., Santi, Peter A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Short Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7881691/
https://www.ncbi.nlm.nih.gov/pubmed/33579352
http://dx.doi.org/10.1186/s13287-021-02184-1
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author Steevens, Aleta R.
Griesbach, Matthew W.
You, Yun
Dutton, James R.
Low, Walter C.
Santi, Peter A.
author_facet Steevens, Aleta R.
Griesbach, Matthew W.
You, Yun
Dutton, James R.
Low, Walter C.
Santi, Peter A.
author_sort Steevens, Aleta R.
collection PubMed
description This research is the first to produce induced pluripotent stem cell-derived inner ear sensory neurons in the Neurog1(+/−) heterozygote mouse using blastocyst complementation. Additionally, this approach corrected non-sensory deficits associated with Neurog1 heterozygosity, indicating that complementation is specific to endogenous Neurog1 function. This work validates the use of blastocyst complementation as a tool to create novel insight into the function of developmental genes and highlights blastocyst complementation as a potential platform for generating chimeric inner ear cell types that can be transplanted into damaged inner ears to improve hearing. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13287-021-02184-1.
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spelling pubmed-78816912021-02-17 Generation of inner ear sensory neurons using blastocyst complementation in a Neurog1(+/−)−deficient mouse Steevens, Aleta R. Griesbach, Matthew W. You, Yun Dutton, James R. Low, Walter C. Santi, Peter A. Stem Cell Res Ther Short Report This research is the first to produce induced pluripotent stem cell-derived inner ear sensory neurons in the Neurog1(+/−) heterozygote mouse using blastocyst complementation. Additionally, this approach corrected non-sensory deficits associated with Neurog1 heterozygosity, indicating that complementation is specific to endogenous Neurog1 function. This work validates the use of blastocyst complementation as a tool to create novel insight into the function of developmental genes and highlights blastocyst complementation as a potential platform for generating chimeric inner ear cell types that can be transplanted into damaged inner ears to improve hearing. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13287-021-02184-1. BioMed Central 2021-02-12 /pmc/articles/PMC7881691/ /pubmed/33579352 http://dx.doi.org/10.1186/s13287-021-02184-1 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Short Report
Steevens, Aleta R.
Griesbach, Matthew W.
You, Yun
Dutton, James R.
Low, Walter C.
Santi, Peter A.
Generation of inner ear sensory neurons using blastocyst complementation in a Neurog1(+/−)−deficient mouse
title Generation of inner ear sensory neurons using blastocyst complementation in a Neurog1(+/−)−deficient mouse
title_full Generation of inner ear sensory neurons using blastocyst complementation in a Neurog1(+/−)−deficient mouse
title_fullStr Generation of inner ear sensory neurons using blastocyst complementation in a Neurog1(+/−)−deficient mouse
title_full_unstemmed Generation of inner ear sensory neurons using blastocyst complementation in a Neurog1(+/−)−deficient mouse
title_short Generation of inner ear sensory neurons using blastocyst complementation in a Neurog1(+/−)−deficient mouse
title_sort generation of inner ear sensory neurons using blastocyst complementation in a neurog1(+/−)−deficient mouse
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7881691/
https://www.ncbi.nlm.nih.gov/pubmed/33579352
http://dx.doi.org/10.1186/s13287-021-02184-1
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