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Relative versus absolute RNA quantification: a comparative analysis based on the example of endothelial expression of vasoactive receptors
BACKGROUND: In the present study, two distinct PCR methods were used for the quantification of genetic material and their results were compared: real-time-PCR (qPCR; relative quantification) and droplet digital PCR (ddPCR; absolute quantification). The comparison of the qPCR and the ddPCR was based...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7883443/ https://www.ncbi.nlm.nih.gov/pubmed/33583396 http://dx.doi.org/10.1186/s12575-021-00144-w |