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Relative versus absolute RNA quantification: a comparative analysis based on the example of endothelial expression of vasoactive receptors

BACKGROUND: In the present study, two distinct PCR methods were used for the quantification of genetic material and their results were compared: real-time-PCR (qPCR; relative quantification) and droplet digital PCR (ddPCR; absolute quantification). The comparison of the qPCR and the ddPCR was based...

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Detalles Bibliográficos
Autores principales: Kuhlmann, Kevin, Cieselski, Melanie, Schumann, Julia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7883443/
https://www.ncbi.nlm.nih.gov/pubmed/33583396
http://dx.doi.org/10.1186/s12575-021-00144-w