Design, Development and Optimization of a Functional Mammalian Cell-Free Protein Synthesis Platform

In this paper, we describe the stepwise development of a cell-free protein synthesis (CFPS) platform derived from cultured Chinese hamster ovary (CHO) cells. We provide a retrospective summary of the design challenges we faced, and the optimized methods developed for the cultivation of cells and the...

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Detalles Bibliográficos
Autores principales: Heide, Chiara, Buldum, Gizem, Moya-Ramirez, Ignacio, Ces, Oscar, Kontoravdi, Cleo, Polizzi, Karen M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Bioengineering and Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7884609/
https://www.ncbi.nlm.nih.gov/pubmed/33604330
http://dx.doi.org/10.3389/fbioe.2020.604091

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7884609/
https://www.ncbi.nlm.nih.gov/pubmed/33604330
http://dx.doi.org/10.3389/fbioe.2020.604091

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