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Design, Development and Optimization of a Functional Mammalian Cell-Free Protein Synthesis Platform
In this paper, we describe the stepwise development of a cell-free protein synthesis (CFPS) platform derived from cultured Chinese hamster ovary (CHO) cells. We provide a retrospective summary of the design challenges we faced, and the optimized methods developed for the cultivation of cells and the...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Frontiers Media S.A.
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7884609/ https://www.ncbi.nlm.nih.gov/pubmed/33604330 http://dx.doi.org/10.3389/fbioe.2020.604091 |