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Isolation of human ESC-derived cardiac derivatives and embryonic heart cells for population and single-cell RNA-seq analysis

The combination of population and single-cell RNA sequencing analysis using human embryonic stem cell (hESC) differentiation and developmental tissues is a powerful approach to elucidate an organ-specific cellular and molecular atlas in human embryogenesis. This protocol describes (1) cardiac-direct...

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Detalles Bibliográficos
Autores principales: Santoro, Federica, Chien, Kenneth R., Sahara, Makoto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7887647/
https://www.ncbi.nlm.nih.gov/pubmed/33644774
http://dx.doi.org/10.1016/j.xpro.2021.100339