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Isolation of human ESC-derived cardiac derivatives and embryonic heart cells for population and single-cell RNA-seq analysis
The combination of population and single-cell RNA sequencing analysis using human embryonic stem cell (hESC) differentiation and developmental tissues is a powerful approach to elucidate an organ-specific cellular and molecular atlas in human embryogenesis. This protocol describes (1) cardiac-direct...
Autores principales: | Santoro, Federica, Chien, Kenneth R., Sahara, Makoto |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7887647/ https://www.ncbi.nlm.nih.gov/pubmed/33644774 http://dx.doi.org/10.1016/j.xpro.2021.100339 |
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