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Glucocerebrosidase reduces the spread of protein aggregation in a Drosophila melanogaster model of neurodegeneration by regulating proteins trafficked by extracellular vesicles

Abnormal protein aggregation within neurons is a key pathologic feature of Parkinson’s disease (PD). The spread of brain protein aggregates is associated with clinical disease progression, but how this occurs remains unclear. Mutations in glucosidase, beta acid 1 (GBA), which encodes glucocerebrosid...

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Autores principales: Jewett, Kathryn A., Thomas, Ruth E., Phan, Chi Q., Lin, Bernice, Milstein, Gillian, Yu, Selina, Bettcher, Lisa F., Neto, Fausto Carnevale, Djukovic, Danijel, Raftery, Daniel, Pallanck, Leo J., Davis, Marie Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7888665/
https://www.ncbi.nlm.nih.gov/pubmed/33539341
http://dx.doi.org/10.1371/journal.pgen.1008859
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author Jewett, Kathryn A.
Thomas, Ruth E.
Phan, Chi Q.
Lin, Bernice
Milstein, Gillian
Yu, Selina
Bettcher, Lisa F.
Neto, Fausto Carnevale
Djukovic, Danijel
Raftery, Daniel
Pallanck, Leo J.
Davis, Marie Y.
author_facet Jewett, Kathryn A.
Thomas, Ruth E.
Phan, Chi Q.
Lin, Bernice
Milstein, Gillian
Yu, Selina
Bettcher, Lisa F.
Neto, Fausto Carnevale
Djukovic, Danijel
Raftery, Daniel
Pallanck, Leo J.
Davis, Marie Y.
author_sort Jewett, Kathryn A.
collection PubMed
description Abnormal protein aggregation within neurons is a key pathologic feature of Parkinson’s disease (PD). The spread of brain protein aggregates is associated with clinical disease progression, but how this occurs remains unclear. Mutations in glucosidase, beta acid 1 (GBA), which encodes glucocerebrosidase (GCase), are the most penetrant common genetic risk factor for PD and dementia with Lewy bodies and associate with faster disease progression. To explore how GBA mutations influence pathogenesis, we previously created a Drosophila model of GBA deficiency (Gba1b) that manifests neurodegeneration and accelerated protein aggregation. Proteomic analysis of Gba1b mutants revealed dysregulation of proteins involved in extracellular vesicle (EV) biology, and we found altered protein composition of EVs from Gba1b mutants. Accordingly, we hypothesized that GBA may influence pathogenic protein aggregate spread via EVs. We found that accumulation of ubiquitinated proteins and Ref(2)P, Drosophila homologue of mammalian p62, were reduced in muscle and brain tissue of Gba1b flies by ectopic expression of wildtype GCase in muscle. Neuronal GCase expression also rescued protein aggregation both cell-autonomously in brain and non-cell-autonomously in muscle. Muscle-specific GBA expression reduced the elevated levels of EV-intrinsic proteins and Ref(2)P found in EVs from Gba1b flies. Perturbing EV biogenesis through neutral sphingomyelinase (nSMase), an enzyme important for EV release and ceramide metabolism, enhanced protein aggregation when knocked down in muscle, but did not modify Gba1b mutant protein aggregation when knocked down in neurons. Lipidomic analysis of nSMase knockdown on ceramide and glucosylceramide levels suggested that Gba1b mutant protein aggregation may depend on relative depletion of specific ceramide species often enriched in EVs. Finally, we identified ectopically expressed GCase within isolated EVs. Together, our findings suggest that GCase deficiency promotes accelerated protein aggregate spread between cells and tissues via dysregulated EVs, and EV-mediated trafficking of GCase may partially account for the reduction in aggregate spread.
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spelling pubmed-78886652021-02-25 Glucocerebrosidase reduces the spread of protein aggregation in a Drosophila melanogaster model of neurodegeneration by regulating proteins trafficked by extracellular vesicles Jewett, Kathryn A. Thomas, Ruth E. Phan, Chi Q. Lin, Bernice Milstein, Gillian Yu, Selina Bettcher, Lisa F. Neto, Fausto Carnevale Djukovic, Danijel Raftery, Daniel Pallanck, Leo J. Davis, Marie Y. PLoS Genet Research Article Abnormal protein aggregation within neurons is a key pathologic feature of Parkinson’s disease (PD). The spread of brain protein aggregates is associated with clinical disease progression, but how this occurs remains unclear. Mutations in glucosidase, beta acid 1 (GBA), which encodes glucocerebrosidase (GCase), are the most penetrant common genetic risk factor for PD and dementia with Lewy bodies and associate with faster disease progression. To explore how GBA mutations influence pathogenesis, we previously created a Drosophila model of GBA deficiency (Gba1b) that manifests neurodegeneration and accelerated protein aggregation. Proteomic analysis of Gba1b mutants revealed dysregulation of proteins involved in extracellular vesicle (EV) biology, and we found altered protein composition of EVs from Gba1b mutants. Accordingly, we hypothesized that GBA may influence pathogenic protein aggregate spread via EVs. We found that accumulation of ubiquitinated proteins and Ref(2)P, Drosophila homologue of mammalian p62, were reduced in muscle and brain tissue of Gba1b flies by ectopic expression of wildtype GCase in muscle. Neuronal GCase expression also rescued protein aggregation both cell-autonomously in brain and non-cell-autonomously in muscle. Muscle-specific GBA expression reduced the elevated levels of EV-intrinsic proteins and Ref(2)P found in EVs from Gba1b flies. Perturbing EV biogenesis through neutral sphingomyelinase (nSMase), an enzyme important for EV release and ceramide metabolism, enhanced protein aggregation when knocked down in muscle, but did not modify Gba1b mutant protein aggregation when knocked down in neurons. Lipidomic analysis of nSMase knockdown on ceramide and glucosylceramide levels suggested that Gba1b mutant protein aggregation may depend on relative depletion of specific ceramide species often enriched in EVs. Finally, we identified ectopically expressed GCase within isolated EVs. Together, our findings suggest that GCase deficiency promotes accelerated protein aggregate spread between cells and tissues via dysregulated EVs, and EV-mediated trafficking of GCase may partially account for the reduction in aggregate spread. Public Library of Science 2021-02-04 /pmc/articles/PMC7888665/ /pubmed/33539341 http://dx.doi.org/10.1371/journal.pgen.1008859 Text en © 2021 Jewett et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Jewett, Kathryn A.
Thomas, Ruth E.
Phan, Chi Q.
Lin, Bernice
Milstein, Gillian
Yu, Selina
Bettcher, Lisa F.
Neto, Fausto Carnevale
Djukovic, Danijel
Raftery, Daniel
Pallanck, Leo J.
Davis, Marie Y.
Glucocerebrosidase reduces the spread of protein aggregation in a Drosophila melanogaster model of neurodegeneration by regulating proteins trafficked by extracellular vesicles
title Glucocerebrosidase reduces the spread of protein aggregation in a Drosophila melanogaster model of neurodegeneration by regulating proteins trafficked by extracellular vesicles
title_full Glucocerebrosidase reduces the spread of protein aggregation in a Drosophila melanogaster model of neurodegeneration by regulating proteins trafficked by extracellular vesicles
title_fullStr Glucocerebrosidase reduces the spread of protein aggregation in a Drosophila melanogaster model of neurodegeneration by regulating proteins trafficked by extracellular vesicles
title_full_unstemmed Glucocerebrosidase reduces the spread of protein aggregation in a Drosophila melanogaster model of neurodegeneration by regulating proteins trafficked by extracellular vesicles
title_short Glucocerebrosidase reduces the spread of protein aggregation in a Drosophila melanogaster model of neurodegeneration by regulating proteins trafficked by extracellular vesicles
title_sort glucocerebrosidase reduces the spread of protein aggregation in a drosophila melanogaster model of neurodegeneration by regulating proteins trafficked by extracellular vesicles
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7888665/
https://www.ncbi.nlm.nih.gov/pubmed/33539341
http://dx.doi.org/10.1371/journal.pgen.1008859
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