Thermodynamic evaluation of the impact of DNA mismatches in PCR-type SARS-CoV-2 primers and probes

BACKGROUND: DNA mismatches can affect the efficiency of PCR techniques if the intended target has mismatches in primer or probe regions. The accepted rule is that mismatches are detrimental as they reduce the hybridization temperatures, yet a more quantitative assessment is rarely performed. METHODS...

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Detalles Bibliográficos
Autores principales: Miranda, Pâmella, Weber, Gerald
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Ltd. 2021
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7888997/
https://www.ncbi.nlm.nih.gov/pubmed/33609730
http://dx.doi.org/10.1016/j.mcp.2021.101707

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7888997/
https://www.ncbi.nlm.nih.gov/pubmed/33609730
http://dx.doi.org/10.1016/j.mcp.2021.101707

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