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Thermodynamic evaluation of the impact of DNA mismatches in PCR-type SARS-CoV-2 primers and probes
BACKGROUND: DNA mismatches can affect the efficiency of PCR techniques if the intended target has mismatches in primer or probe regions. The accepted rule is that mismatches are detrimental as they reduce the hybridization temperatures, yet a more quantitative assessment is rarely performed. METHODS...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Elsevier Ltd.
2021
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7888997/ https://www.ncbi.nlm.nih.gov/pubmed/33609730 http://dx.doi.org/10.1016/j.mcp.2021.101707 |