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FLICK: an optimized plate reader-based assay to infer cell death kinetics

Evaluating drug sensitivity is improved by directly quantifying death kinetics, rather than correlates of viability, such as metabolic activity. This is challenging, requiring time-lapse microscopy and genetically encoded labels to distinguish live and dead cells. Here, we describe fluorescence-base...

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Detalles Bibliográficos
Autores principales: Richards, Ryan, Honeywell, Megan E., Lee, Michael J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890003/
https://www.ncbi.nlm.nih.gov/pubmed/33659903
http://dx.doi.org/10.1016/j.xpro.2021.100327