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High-Level Patchoulol Biosynthesis in Artemisia annua L.
Terpenes constitute the largest class of secondary metabolites in plants. Some terpenes are essential for plant growth and development, membrane components, and photosynthesis. Terpenes are also economically useful for industry, agriculture, and pharmaceuticals. However, there is very low content of...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890116/ https://www.ncbi.nlm.nih.gov/pubmed/33614607 http://dx.doi.org/10.3389/fbioe.2020.621127 |
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author | Fu, Xueqing Zhang, Fangyuan Ma, Yanan Hassani, Danial Peng, Bowen Pan, Qifang Zhang, Yuhua Deng, Zhongxiang Liu, Wenbo Zhang, Jixiu Han, Lei Chen, Dongfang Zhao, Jingya Li, Ling Sun, Xiaofen Tang, Kexuan |
author_facet | Fu, Xueqing Zhang, Fangyuan Ma, Yanan Hassani, Danial Peng, Bowen Pan, Qifang Zhang, Yuhua Deng, Zhongxiang Liu, Wenbo Zhang, Jixiu Han, Lei Chen, Dongfang Zhao, Jingya Li, Ling Sun, Xiaofen Tang, Kexuan |
author_sort | Fu, Xueqing |
collection | PubMed |
description | Terpenes constitute the largest class of secondary metabolites in plants. Some terpenes are essential for plant growth and development, membrane components, and photosynthesis. Terpenes are also economically useful for industry, agriculture, and pharmaceuticals. However, there is very low content of most terpenes in microbes and plants. Chemical or microbial synthesis of terpenes are often costly. Plants have the elaborate and economic biosynthetic way of producing high-value terpenes through photosynthesis. Here we engineered the heterogenous sesquiterpenoid patchoulol production in A. annua. When using a strong promoter such as 35S to over express the avian farnesyl diphosphate synthase gene and patchoulol synthase gene, the highest content of patchoulol was 52.58 μg/g DW in transgenic plants. When altering the subcellular location of the introduced sesquiterpene synthetase via a signal peptide, the accumulation of patchoulol was observably increased to 273 μg/g DW. This case demonstrates that A. annua plant with glandular trichomes is a useful platform for synthetic biology studies. |
format | Online Article Text |
id | pubmed-7890116 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-78901162021-02-19 High-Level Patchoulol Biosynthesis in Artemisia annua L. Fu, Xueqing Zhang, Fangyuan Ma, Yanan Hassani, Danial Peng, Bowen Pan, Qifang Zhang, Yuhua Deng, Zhongxiang Liu, Wenbo Zhang, Jixiu Han, Lei Chen, Dongfang Zhao, Jingya Li, Ling Sun, Xiaofen Tang, Kexuan Front Bioeng Biotechnol Bioengineering and Biotechnology Terpenes constitute the largest class of secondary metabolites in plants. Some terpenes are essential for plant growth and development, membrane components, and photosynthesis. Terpenes are also economically useful for industry, agriculture, and pharmaceuticals. However, there is very low content of most terpenes in microbes and plants. Chemical or microbial synthesis of terpenes are often costly. Plants have the elaborate and economic biosynthetic way of producing high-value terpenes through photosynthesis. Here we engineered the heterogenous sesquiterpenoid patchoulol production in A. annua. When using a strong promoter such as 35S to over express the avian farnesyl diphosphate synthase gene and patchoulol synthase gene, the highest content of patchoulol was 52.58 μg/g DW in transgenic plants. When altering the subcellular location of the introduced sesquiterpene synthetase via a signal peptide, the accumulation of patchoulol was observably increased to 273 μg/g DW. This case demonstrates that A. annua plant with glandular trichomes is a useful platform for synthetic biology studies. Frontiers Media S.A. 2021-02-04 /pmc/articles/PMC7890116/ /pubmed/33614607 http://dx.doi.org/10.3389/fbioe.2020.621127 Text en Copyright © 2021 Fu, Zhang, Ma, Hassani, Peng, Pan, Zhang, Deng, Liu, Zhang, Han, Chen, Zhao, Li, Sun and Tang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology Fu, Xueqing Zhang, Fangyuan Ma, Yanan Hassani, Danial Peng, Bowen Pan, Qifang Zhang, Yuhua Deng, Zhongxiang Liu, Wenbo Zhang, Jixiu Han, Lei Chen, Dongfang Zhao, Jingya Li, Ling Sun, Xiaofen Tang, Kexuan High-Level Patchoulol Biosynthesis in Artemisia annua L. |
title | High-Level Patchoulol Biosynthesis in Artemisia annua L. |
title_full | High-Level Patchoulol Biosynthesis in Artemisia annua L. |
title_fullStr | High-Level Patchoulol Biosynthesis in Artemisia annua L. |
title_full_unstemmed | High-Level Patchoulol Biosynthesis in Artemisia annua L. |
title_short | High-Level Patchoulol Biosynthesis in Artemisia annua L. |
title_sort | high-level patchoulol biosynthesis in artemisia annua l. |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890116/ https://www.ncbi.nlm.nih.gov/pubmed/33614607 http://dx.doi.org/10.3389/fbioe.2020.621127 |
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