Cargando…

High-Level Patchoulol Biosynthesis in Artemisia annua L.

Terpenes constitute the largest class of secondary metabolites in plants. Some terpenes are essential for plant growth and development, membrane components, and photosynthesis. Terpenes are also economically useful for industry, agriculture, and pharmaceuticals. However, there is very low content of...

Descripción completa

Detalles Bibliográficos
Autores principales: Fu, Xueqing, Zhang, Fangyuan, Ma, Yanan, Hassani, Danial, Peng, Bowen, Pan, Qifang, Zhang, Yuhua, Deng, Zhongxiang, Liu, Wenbo, Zhang, Jixiu, Han, Lei, Chen, Dongfang, Zhao, Jingya, Li, Ling, Sun, Xiaofen, Tang, Kexuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890116/
https://www.ncbi.nlm.nih.gov/pubmed/33614607
http://dx.doi.org/10.3389/fbioe.2020.621127
_version_ 1783652448244596736
author Fu, Xueqing
Zhang, Fangyuan
Ma, Yanan
Hassani, Danial
Peng, Bowen
Pan, Qifang
Zhang, Yuhua
Deng, Zhongxiang
Liu, Wenbo
Zhang, Jixiu
Han, Lei
Chen, Dongfang
Zhao, Jingya
Li, Ling
Sun, Xiaofen
Tang, Kexuan
author_facet Fu, Xueqing
Zhang, Fangyuan
Ma, Yanan
Hassani, Danial
Peng, Bowen
Pan, Qifang
Zhang, Yuhua
Deng, Zhongxiang
Liu, Wenbo
Zhang, Jixiu
Han, Lei
Chen, Dongfang
Zhao, Jingya
Li, Ling
Sun, Xiaofen
Tang, Kexuan
author_sort Fu, Xueqing
collection PubMed
description Terpenes constitute the largest class of secondary metabolites in plants. Some terpenes are essential for plant growth and development, membrane components, and photosynthesis. Terpenes are also economically useful for industry, agriculture, and pharmaceuticals. However, there is very low content of most terpenes in microbes and plants. Chemical or microbial synthesis of terpenes are often costly. Plants have the elaborate and economic biosynthetic way of producing high-value terpenes through photosynthesis. Here we engineered the heterogenous sesquiterpenoid patchoulol production in A. annua. When using a strong promoter such as 35S to over express the avian farnesyl diphosphate synthase gene and patchoulol synthase gene, the highest content of patchoulol was 52.58 μg/g DW in transgenic plants. When altering the subcellular location of the introduced sesquiterpene synthetase via a signal peptide, the accumulation of patchoulol was observably increased to 273 μg/g DW. This case demonstrates that A. annua plant with glandular trichomes is a useful platform for synthetic biology studies.
format Online
Article
Text
id pubmed-7890116
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-78901162021-02-19 High-Level Patchoulol Biosynthesis in Artemisia annua L. Fu, Xueqing Zhang, Fangyuan Ma, Yanan Hassani, Danial Peng, Bowen Pan, Qifang Zhang, Yuhua Deng, Zhongxiang Liu, Wenbo Zhang, Jixiu Han, Lei Chen, Dongfang Zhao, Jingya Li, Ling Sun, Xiaofen Tang, Kexuan Front Bioeng Biotechnol Bioengineering and Biotechnology Terpenes constitute the largest class of secondary metabolites in plants. Some terpenes are essential for plant growth and development, membrane components, and photosynthesis. Terpenes are also economically useful for industry, agriculture, and pharmaceuticals. However, there is very low content of most terpenes in microbes and plants. Chemical or microbial synthesis of terpenes are often costly. Plants have the elaborate and economic biosynthetic way of producing high-value terpenes through photosynthesis. Here we engineered the heterogenous sesquiterpenoid patchoulol production in A. annua. When using a strong promoter such as 35S to over express the avian farnesyl diphosphate synthase gene and patchoulol synthase gene, the highest content of patchoulol was 52.58 μg/g DW in transgenic plants. When altering the subcellular location of the introduced sesquiterpene synthetase via a signal peptide, the accumulation of patchoulol was observably increased to 273 μg/g DW. This case demonstrates that A. annua plant with glandular trichomes is a useful platform for synthetic biology studies. Frontiers Media S.A. 2021-02-04 /pmc/articles/PMC7890116/ /pubmed/33614607 http://dx.doi.org/10.3389/fbioe.2020.621127 Text en Copyright © 2021 Fu, Zhang, Ma, Hassani, Peng, Pan, Zhang, Deng, Liu, Zhang, Han, Chen, Zhao, Li, Sun and Tang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Fu, Xueqing
Zhang, Fangyuan
Ma, Yanan
Hassani, Danial
Peng, Bowen
Pan, Qifang
Zhang, Yuhua
Deng, Zhongxiang
Liu, Wenbo
Zhang, Jixiu
Han, Lei
Chen, Dongfang
Zhao, Jingya
Li, Ling
Sun, Xiaofen
Tang, Kexuan
High-Level Patchoulol Biosynthesis in Artemisia annua L.
title High-Level Patchoulol Biosynthesis in Artemisia annua L.
title_full High-Level Patchoulol Biosynthesis in Artemisia annua L.
title_fullStr High-Level Patchoulol Biosynthesis in Artemisia annua L.
title_full_unstemmed High-Level Patchoulol Biosynthesis in Artemisia annua L.
title_short High-Level Patchoulol Biosynthesis in Artemisia annua L.
title_sort high-level patchoulol biosynthesis in artemisia annua l.
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890116/
https://www.ncbi.nlm.nih.gov/pubmed/33614607
http://dx.doi.org/10.3389/fbioe.2020.621127
work_keys_str_mv AT fuxueqing highlevelpatchoulolbiosynthesisinartemisiaannual
AT zhangfangyuan highlevelpatchoulolbiosynthesisinartemisiaannual
AT mayanan highlevelpatchoulolbiosynthesisinartemisiaannual
AT hassanidanial highlevelpatchoulolbiosynthesisinartemisiaannual
AT pengbowen highlevelpatchoulolbiosynthesisinartemisiaannual
AT panqifang highlevelpatchoulolbiosynthesisinartemisiaannual
AT zhangyuhua highlevelpatchoulolbiosynthesisinartemisiaannual
AT dengzhongxiang highlevelpatchoulolbiosynthesisinartemisiaannual
AT liuwenbo highlevelpatchoulolbiosynthesisinartemisiaannual
AT zhangjixiu highlevelpatchoulolbiosynthesisinartemisiaannual
AT hanlei highlevelpatchoulolbiosynthesisinartemisiaannual
AT chendongfang highlevelpatchoulolbiosynthesisinartemisiaannual
AT zhaojingya highlevelpatchoulolbiosynthesisinartemisiaannual
AT liling highlevelpatchoulolbiosynthesisinartemisiaannual
AT sunxiaofen highlevelpatchoulolbiosynthesisinartemisiaannual
AT tangkexuan highlevelpatchoulolbiosynthesisinartemisiaannual