Cargando…

Systematic improvements in lentiviral transduction of primary human natural killer cells undergoing ex vivo expansion

Transduction of primary human natural killer (NK) cells with lentiviral vectors has historically been challenging. We sought to evaluate multiple parameters to optimize lentiviral transduction of human peripheral blood NK cells being expanded to large numbers using a good manufacturing practice (GMP...

Descripción completa

Detalles Bibliográficos
Autores principales:	Allan, David S.J., Chakraborty, Mala, Waller, Giacomo C., Hochman, Michael J., Poolcharoen, Akkapon, Reger, Robert N., Childs, Richard W.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society of Gene & Cell Therapy 2021
Materias:	Original Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7890427/ https://www.ncbi.nlm.nih.gov/pubmed/33665226 http://dx.doi.org/10.1016/j.omtm.2021.01.008

Ejemplares similares

Optimized protocols for γδ T cell expansion and lentiviral transduction
por: Wang, Rui-Ning, et al.
Publicado: (2019)

Highly efficient ex vivo lentiviral transduction of primary human pancreatic exocrine cells
por: Balak, Jeetindra R. A., et al.
Publicado: (2019)

High-affinity CD16 integration into a CRISPR/Cas9-edited CD38 locus augments CD38-directed antitumor activity of primary human natural killer cells
por: Clara, Joseph Andrew, et al.
Publicado: (2022)

Process for an efficient lentiviral cell transduction
por: Pirona, Anna Chiara, et al.
Publicado: (2020)

Cardiovascular Manifestation of the BNT162b2 mRNA COVID-19 Vaccine in Adolescents
por: Mansanguan, Suyanee, et al.
Publicado: (2022)

Driving DNA transposition by lentiviral protein transduction
por: Cai, Yujia, et al.
Publicado: (2014)

Human Platelet Lysate Media Supplement Supports Lentiviral Transduction and Expansion of Human T Lymphocytes While Maintaining Memory Phenotype
por: Canestrari, Emanuele, et al.
Publicado: (2019)

Avoiding lentiviral transduction culture induced MSC senescence
por: Pan, Yung-Wei, et al.
Publicado: (2009)

Vpx mediated degradation of SAMHD1 has only a very limited effect on lentiviral transduction rate in ex vivo cultured HSPCs()
por: Li, Duo, et al.
Publicado: (2015)

Assessing the Impact of Cyclosporin A on Lentiviral Transduction and Preservation of Human Hematopoietic Stem Cells in Clinically Relevant Ex Vivo Gene Therapy Settings
por: Petrillo, Carolina, et al.
Publicado: (2019)

Transduction of Primary AML Cells with Lentiviral Vector for In Vitro Study or In Vivo Engraftment
por: Schimmer, Aaron D., et al.
Publicado: (2020)

Minimal impact of ZAP on lentiviral vector production and transduction efficiency
por: Sertkaya, Helin, et al.
Publicado: (2021)

Cardiac evaluation in adults with dengue virus infection by serial echocardiography
por: Mansanguan, Chayasin, et al.
Publicado: (2021)

Identification of a small molecule for enhancing lentiviral transduction of T cells
por: Malach, Paulina, et al.
Publicado: (2023)

Lentiviral vector transduction of spermatozoa as a tool for the study of early development
por: Chandrashekran, Anil, et al.
Publicado: (2014)

Characterization and ex vivo Expansion of Human Placenta-Derived Natural Killer Cells for Cancer Immunotherapy
por: Kang, Lin, et al.
Publicado: (2013)

Ex Vivo Expansion of Highly Cytotoxic Natural Killer Cells Using Optimal Culture Medium
por: Kim, Seokho
Publicado: (2022)

Engineering fusogenic molecules to achieve targeted transduction of enveloped lentiviral vectors
por: Lei, Yuning, et al.
Publicado: (2009)

Toward Tightly Tuned Gene Expression Following Lentiviral Vector Transduction
por: Page, Audrey, et al.
Publicado: (2020)

Cellular Proteo-Transcriptomic Changes in the Immediate Early-Phase of Lentiviral Transduction
por: Linkner, Tamás Richárd, et al.
Publicado: (2021)

Lentiviral transduction facilitates RNA interference in the nematode parasite Nippostrongylus brasiliensis
por: Hagen, Jana, et al.
Publicado: (2021)

An efficient strategy for generation of transgenic mice by lentiviral transduction of male germline stem cells in vivo
por: Qin, Jinzhou, et al.
Publicado: (2015)

Enhancing Lentiviral and Alpharetroviral Transduction of Human Hematopoietic Stem Cells for Clinical Application
por: Schott, Juliane W., et al.
Publicado: (2019)

Lentiviral delivery of CTLA-4 shRNA improves the expansion of cytokine-induced killer cells and enhances cytotoxic activity in vitro
por: Rui, Tao, et al.
Publicado: (2018)

Brain-targeted ex vivo lentiviral gene therapy: Implications for MPS and beyond
por: Bradbury, Allison M.
Publicado: (2023)

A Nontoxic Transduction Enhancer Enables Highly Efficient Lentiviral Transduction of Primary Murine T Cells and Hematopoietic Stem Cells
por: Delville, Marianne, et al.
Publicado: (2018)

Alteration of T cell immunity by lentiviral transduction of human monocyte-derived dendritic cells
por: Chen, Xiaochuan, et al.
Publicado: (2004)

In situ transduction of stromal cells and thymocytes upon intrathymic injection of lentiviral vectors
por: Marodon, Gilles, et al.
Publicado: (2004)

Polybrene Inhibits Human Mesenchymal Stem Cell Proliferation during Lentiviral Transduction
por: Lin, Paul, et al.
Publicado: (2011)

Lentiviral Transduction of CD34(+) Cells Induces Genome-Wide Epigenetic Modifications
por: Yamagata, Yoshiaki, et al.
Publicado: (2012)

Lentiviral transduction of rat Sertoli cells as a means to modify gene expression
por: Nicholls, Peter K., et al.
Publicado: (2012)

Improving the Transduction of Bone Marrow–Derived Cells with an Integrase-Defective Lentiviral Vector
por: Pay, S. Louise, et al.
Publicado: (2018)

Proteasome Inhibition Increases the Efficiency of Lentiviral Vector-Mediated Transduction of Trabecular Meshwork
por: Aktas, Zeynep, et al.
Publicado: (2018)

DNA transposition by protein transduction of the piggyBac transposase from lentiviral Gag precursors
por: Cai, Yujia, et al.
Publicado: (2014)

Bortezomib Treatment to Potentiate the Anti-tumor Immunity of Ex-vivo Expanded Adoptively Infused Autologous Natural Killer Cells
por: Lundqvist, Andreas, et al.
Publicado: (2011)

Laboratory-Scale Lentiviral Vector Production and Purification for Enhanced Ex Vivo and In Vivo Genetic Engineering
por: Soldi, Monica, et al.
Publicado: (2020)

Immunization of Mice with Lentiviral Vectors Targeted to MHC Class II+ Cells Is Due to Preferential Transduction of Dendritic Cells In Vivo
por: Ciré, Séverine, et al.
Publicado: (2014)

Ex vivo expansion of human peripheral blood natural killer cells and cytotoxic T lymphocytes from lung cancer patients
por: Nhung, Hoang Thi My, et al.
Publicado: (2018)

Efficient delivery of Cre-recombinase to neurons in vivo and stable transduction of neurons using adeno-associated and lentiviral vectors
por: Ahmed, Bushra Y, et al.
Publicado: (2004)

Therapeutic protein transduction of mammalian cells and mice by nucleic acid-free lentiviral nanoparticles
por: Link, Nils, et al.
Publicado: (2006)

Cannot write session to /tmp/vufind_sessions/sess_ke1dlt8h8h82vnplhhgd7shb96